Cloning and expression of a geranylgeranyl diphosphate synthase gene: insights into the synthesis of termite defence secretion
Cloning and expression of a geranylgeranyl diphosphate synthase gene: insights into the synthesis of termite defence secretion
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DOI：
10.1111/j.1365-2583.2007.00709.x
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发表时间：
2007-02-01
影响因子：
2.6
通讯作者：
Miura, Toru
中科院分区：
文献类型：
--
作者：
Hojo, Masaru;Matsumoto, Tadao;Miura, Toru
在Nasutitermes takasagoensis（一种士兵进行专门化学防御的白蚁）中，Nts19-1基因仅在士兵头部高度表达。在本研究中，获得了该基因的两种类型的转录本，并通过cDNA末端快速扩增（RACE）确定了全长cDNA。这些转录物被假定为香叶基香叶基二磷酸 (GGPP) 合酶基因的同源物，参与类异戊二烯生物合成途径中二甲基烯丙基二磷酸与异戊烯基二磷酸的缩合。因此这些基因被称为 NtGGPPS1。 GGPP是植物中二萜的前体。原位杂交将NtGGPPS1表达定位于产生多种二萜的额腺库的表皮分泌细胞，表明NtGGPPS1参与防御分泌的生物合成。
In Nasutitermes takasagoensis, a termite in which soldiers perform specialized chemical defence, Nts19-1 gene is highly expressed exclusively in soldier head. In this study, two types of transcripts for this gene were obtained, and the full-length cDNAs were determined by rapid amplification of cDNA ends (RACE). These transcripts were putative homologues of the geranylgeranyl diphosphate (GGPP) synthase gene, involved in the condensation of dimethylallyl diphosphate with isopentenyl diphosphate in the isoprenoid biosynthetic pathway. The genes were thus termed NtGGPPS1. GGPP is a precursor of diterpenes in plants. In situ hybridization localized NtGGPPS1 expression to the epidermal secretory cells of the frontal gland reservoir where many kinds of diterpenes are produced, suggesting that NtGGPPS1 is involved in the biosynthesis of defence secretion.