Parameters affecting the inhibition of Candida albicans GDH 2023 and GRI 2773 blastospore viability by purified synthetic salivary histidine-rich polypeptides.
Parameters affecting the inhibition of Candida albicans GDH 2023 and GRI 2773 blastospore viability by purified synthetic salivary histidine-rich polypeptides.
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影响纯化的合成唾液富含组氨酸多肽对白色念珠菌 GDH 2023 和 GRI 2773 芽生孢子活力的抑制的参数。
DOI：
10.1111/j.1399-302x.1990.tb00651.x
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发表时间：
1990
影响因子：
--
通讯作者：
Pollock,JJ
中科院分区：
文献类型：
--
作者：
Santarpia3rd,RP;Cho,MI;Pollock,JJ
纯化的富含唾液组氨酸的多肽HRP2、3、4、5和6对白念珠菌芽胞活力有抑制作用，其酵母细胞浓度为10~10~6个/ml，其中HRP-4的抑制率最高，当多肽浓度为0.5μg/ml时具有显著的杀灭活性。相反，在非生长条件下测试的pH范围内，杀灭作用不会发生变化。电子显微镜结果显示，在pH为5时，HRP损伤似乎是从细胞膜开始的。在pH 7.4时，显微照片显示细胞内破坏的明显证据表明，与酸性pH相比，中性条件下的损伤更广泛。这些结果表明，在不断变化的口腔领域，高密度脂蛋白有望成为白色念珠菌的有效杀手。
Purified synthetic salivary histidine‐rich polypeptides, HRPs 2, 3, 4, 5, and 6, were observed to inhibit Candida albicans blastospore viability at yeast cell concentrations ranging from 102 to greater than 106 colony forming units per ml. Among the HRPs, HRP‐4 was the best inhibitor with significant killing activity noted at a peptide concentration of 0.5 μg per ml. Antifungal potency under growth conditions was observed to be dependent upon pH. In contrast, killing did not vary throughout the pH range tested under non‐growth conditions. Electron microscopy results demonstrated HRP damage at pH 5 which appeared to be initiated at the membrane. At pH 7.4, micrographs revealed clear evidence of intracellular destruction suggesting more extensive damage at neutral as compared to acidic pH. These results suggest that within the changing realm of the oral cavity, the HRPs would be expected to be potent killers of C. albicans.