DISCRIMINATION OF VARIOUS EPITHELIA BY SIMPLE MORPHOMETRIC EVALUATION OF THE BASAL-CELL LAYER - A LIGHT MICROSCOPIC ANALYSIS OF PSEUDOSTRATIFIED, METAPLASTIC AND DYSPLASTIC NASAL EPITHELIUM IN NICKEL WORKERS
DISCRIMINATION OF VARIOUS EPITHELIA BY SIMPLE MORPHOMETRIC EVALUATION OF THE BASAL-CELL LAYER - A LIGHT MICROSCOPIC ANALYSIS OF PSEUDOSTRATIFIED, METAPLASTIC AND DYSPLASTIC NASAL EPITHELIUM IN NICKEL WORKERS
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DOI：
10.1007/bf02890380
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
REITH, A
中科院分区：
文献类型：
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作者：
BOYSEN, M;REITH, A
提出了一种简单的形态测量方法，通过仅评估基底层的细胞特征，对假复层、各种类型的化生和发育异常上皮进行客观分类。出于诊断原因，从镍工人的鼻粘膜中取出活检标本（54），并对半薄的甲苯胺蓝染色切片进行分析。区分各种类型上皮的最敏感参数是：横向核直径、核仁大小和基底细胞宽度，以基底层上具有最宽附着面的细胞轮廓加权的指数表示。这 3 个参数的组合可以清楚地区分发育异常、化生和假复层上皮。这些参数从假复层上皮经过两个组织学上可区分的化生阶段（复层立方上皮和混合复层立方/复层鳞状上皮）到完全发育的鳞状上皮，依次增加，支持化生逐渐发展的概念。这些参数从化生到异型增生的不断增加进一步表明化生是鼻上皮异型增生发展的必要步骤。这种形态测量模型在监测小的连续上皮变化方面特别有用，并且也可用于评估其他类型的上皮。
A simple morphometric method is presented for objective classification of pseudostratified, various types of metaplastic, and dysplastic epithelium by evaluation of cellular features in the basal layer only. Biopsy specimens (54) were taken for diagnostic reasons from the nasal mucosa of Ni workers, and semithin toluidine-blue-stained sections were analyzed. The most sensitive parameters in distinguishing between the various types of epithelium were: the transverse nuclear diameter, the size of the nucleoli and the basal cell width expressed as an index weighted towards the cell profiles with the broadest attachment face on the basal lamina. A combination of these 3 parameters allows a clear separation between dysplastic, metaplastic and pseudostratified epithelium. The sequential increase in these parameters from pseudostratified epithelium through 2 histologically distinguishable stages of metaplasia (stratified cuboidal and mixed stratified cuboidal/stratified squamous epithelium) to fully developed squamous epithelium supports the concept that metaplasia develops gradually. The continuous increase in these parameters from metaplasia to dysplasia further suggests that metaplasia is a necessary step in the development of nasal epithelial dysplasia. This morphometric model appears especially useful in monitoring small sequential epithelial changes, and might also be used for evaluating other types of epithelia.