Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo
Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo
复制标题
都柏林念珠菌四种不同基因型的鉴定及体内外微进化检测
DOI：
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发表时间：
2002
影响因子：
9.4
通讯作者：
D. Coleman
中科院分区：
文献类型：
--
作者：
S. Gee;S. Joly;D. Soll;J. Meis;P. Verweij;I. Polacheck;D. Sullivan;D. Coleman
摘要本研究进一步研究了都柏林假丝酵母的种群结构及其微进化能力。使用来自15个国家94名患者的98株分离株（包括80株口服分离株），我们证实了在C种内存在两个不同的种群。dubliniensis，分别命名为Cd 25 Ⅰ组和Cd 25 Ⅱ组。都柏林特异性探针Cd 25。大多数Cd 25 I组分离株（48/71，67.6%）来自人类免疫缺陷病毒（HIV）感染者，而大多数Cd 25 II组分离株（19/27，70.4%）来自HIV阴性个体（P ≤ 0.001）。从19个代表性的分离物的rRNA基因的内部转录间隔区（ITS）区域的核苷酸序列分析显示存在四个独立的基因型。所有的Cd 25组I测试菌株属于基因型1，而Cd 25组II人口是由三个不同的基因型（基因型2至4），这对应于不同的分支内的Cd 25组II人口。这些发现证实了基因型特异性PCR引物与70株。我们还证明了C. Dubliniensis可以在体内和体外表现出与其他酵母物种中发生的微进化。DNA指纹图谱使用C。dubliniensis探针Cd 25、Cd 24和Cd 1以及从来自8名独立患者的相同标本中回收的多个口腔分离物的核型分析揭示了8个克隆群体中的6个的微进化。同样，从两个不同的患者的不同解剖部位连续克隆分离株表现出微进化。当两个对氟康唑敏感的临床分离株在体外暴露于药物时，也显示发生微进化。四种C. dubliniensis基因型和C. dubliniensis经历微进化的可能性尚待确定。
ABSTRACT The present study investigates further the population structure of Candida dubliniensis and its ability to exhibit microevolution. Using 98 isolates (including 80 oral isolates) from 94 patients in 15 countries, we confirmed the existence of two distinct populations within the species C. dubliniensis, designated Cd25 group I and Cd25 group II, respectively, on the basis of DNA fingerprints generated with the C. dubliniensis-specific probe Cd25. The majority of Cd25 group I isolates (48 of 71, 67.6%) were from human immunodeficiency virus (HIV)-infected individuals, whereas the majority of Cd25 group II isolates (19 of 27, 70.4%) were from HIV-negative individuals (P ≤ 0.001). Nucleotide sequence analysis of the internal transcribed spacer (ITS) regions of the rRNA genes from 19 representative isolates revealed the presence of four separate genotypes. All of the Cd25 group I isolates tested belonged to genotype 1, while the Cd25 group II population was comprised of three distinct genotypes (genotypes 2 to 4), which corresponded to distinct clades within the Cd25 group II population. These findings were confirmed using genotype-specific PCR primers with 70 isolates. We also showed that C. dubliniensis can exhibit microevolution in vivo and in vitro as occurs in other yeast species. DNA fingerprinting using the C. dubliniensis probes Cd25, Cd24, and Cd1 and karyotype analysis of multiple oral isolates recovered from the same specimen from each of eight separate patients revealed microevolution in six of eight of the clonal populations. Similarly, sequential clonal isolates from various anatomical sites in two separate patients exhibited microevolution. Microevolution was also shown to occur when two clinical isolates susceptible to fluconazole were exposed to the drug in vitro. The epidemiological significance of the four C. dubliniensis genotypes and the ability of C. dubliniensis to undergo microevolution has yet to be established.
影响因子：
3.8
作者：
TC White;M. Pfaller;M. Rinaldi;J. Smith;S. Redding
通讯作者：
TC White;M. Pfaller;M. Rinaldi;J. Smith;S. Redding
DOI：
10.1099/13500872-145-5-1137
发表时间：
1999-05-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Lott, TJ;Holloway, BP;Arnold, J
通讯作者：
Arnold, J