GUN1 regulates tetrapyrrole biosynthesis
GUN1 regulates tetrapyrrole biosynthesis
复制标题
GUN1 调节四吡咯生物合成
DOI：
--
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
T. Masuda
中科院分区：
文献类型：
--
作者：
Takayuki Shimizu;N. Mochizuki;A. Nagatani;Satoru Watanabe;T. Shimada;Kan Tanaka;Yuuki Hayashi;M. Arai;S. Kacprzak;D. Leister;H. Okamoto;M. J. Terry;T. Masuda
在发育中的叶绿体的光合机构的生物发生需要组装的蛋白质编码的核和叶绿体基因组1。为了协调这一过程，这些细胞器之间需要进行通信，虽然我们对细胞核如何控制叶绿体发育有了很好的了解，但此时叶绿体如何与细胞核进行通信仍然是个未知数。我们所知道的是来自于一系列开创性的工作，其中一系列基因组解偶联（枪）突变体被鉴定出在叶绿体损伤后核基因表达升高3。在六个报道的枪突变中，五个在四吡咯生物合成蛋白4 -6中，这导致了叶绿体到细胞核逆行信号传导模型的发展，其中铁螯合酶1（FC 1）依赖性血红素合成产生促进光合作用相关基因表达的正信号6。然而，最强的枪突变体gun 17的分子后果是未知的，阻止了叶绿体到细胞核信号的统一假设的发展。在这里，我们表明，GUN 1直接绑定到血红素和其他金属卟啉，影响通量通过四吡咯生物合成途径，并可以增加螯合酶活性的FC 1。这些结果提高了GUN 1的信号传导作用可能通过四吡咯代谢的变化表现出来的可能性，并支持四吡咯作为单一生物叶绿体到细胞核逆行信号传导途径的介质的作用。
The biogenesis of the photosynthetic apparatus in developing chloroplasts requires the assembly of proteins encoded on both nuclear and chloroplast genomes1. To co-ordinate this process there needs to be communication between these organelles, and while we have a good understanding of how the nucleus controls chloroplast development, how the chloroplast communicates with the nucleus at this time is still essentially unknown2. What we do know comes from pioneering work in which a series of genomes uncoupled (gun) mutants were identified that show elevated nuclear gene expression after chloroplast damage3. Of the six reported gun mutations, five are in tetrapyrrole biosynthesis proteins4-6 and this has led to the development of a model for chloroplast-to-nucleus retrograde signaling in which ferrochelatase 1 (FC1)-dependent heme synthesis generates a positive signal promoting expression of photosynthesis-related genes6. However, the molecular consequences of the strongest of the gun mutants, gun17, is unknown, preventing the development of a unifying hypothesis for chloroplast-to-nucleus signaling. Here, we show that GUN1 directly binds to heme and other metal-porphyrins, affects flux through the tetrapyrrole biosynthesis pathway and can increase the chelatase activity of FC1. These results raise the possibility that the signaling role of GUN1 may be manifested through changes in tetrapyrrole metabolism and supports a role for tetrapyrroles as mediators of a single biogenic chloroplast-to-nucleus retrograde signaling pathway.
DOI：
10.1111/tpj.12011
发表时间：
2013-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Woodson JD;Perez-Ruiz JM;Schmitz RJ;Ecker JR;Chory J
通讯作者：
Chory J
影响因子：
7.4
作者：
Tadini, Luca;Pesaresi, Paolo;Leister, Dario
通讯作者：
Leister, Dario
影响因子：
2.9
作者：
Hargrove, MS;Barrick, D;Olson, JS
通讯作者：
Olson, JS