The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis.
The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis.
复制标题
果蝇的母体核蛋白对于胚胎发生具有两个重要的不同作用。
DOI：
10.1093/genetics/143.4.1653
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Hashimoto,C
中科院分区：
文献类型：
--
作者：
Hong,CC;Hashimoto,C
果蝇胚胎的背腹极性是母系所必需的。它编码一种不寻常的镶嵌蛋白，具有蛋白酶结构域，可能触发腹部发育所需的蛋白酶级联反应。我们描述了裸突变的表型和分子分析，为裸蛋白功能提供了进一步的见解。令人惊讶的是，nudelmutations主要导致背侧细胞命运扩展到腹侧和侧部细胞命运的背侧胚胎或未能发育超过早期胚胎阶段的脆弱卵子。因此，裸蛋白不仅是胚胎背腹极性所必需的，而且也是卵结构完整性所必需的。nudel等位基因之间的互补和拮抗相互作用表明，nudel蛋白是功能模块化的，蛋白质-蛋白质相互作用是重要的nudel蛋白功能。产生背侧化胚胎的Threenudelmutations映射到nudel的蛋白酶结构域，表明该结构域是定义胚胎背腹极性所必需的。最后，裸等位基因的某些组合同时产生完全背化和正常的胚胎，但很少有中间突变表型的胚胎。这种不寻常的双相表型分布可能表明，裸细胞活性高于阈值是产生胚胎背腹极性所必需的。
Thenudelgene is maternally required to define dorsoventral polarity of the Drosophila embryo. It encodes an unusual mosaic protein with a protease domain that may trigger the protease cascade required for ventral development. We describe phenotypic and molecular analyses ofnudelmutations that provide further insight into nudel protein function. Surprisingly,nudelmutations primarily cause either dorsalized embryos in which dorsal cell fates are expanded over ventral and lateral cell fates or fragile eggs that fail to develop beyond early embryonic stages. The nudel protein is therefore required not only for embryonic dorsoventral polarity but also for structural integrity of the egg. Complementation and antagonistic interactions betweennudelalleles suggest that the nudel protein is functionally modular and that protein-protein interactions are important for nudel protein function. Threenudelmutations that produce dorsalized embryos map to the protease domain of nudel, suggesting that this domain is specifically required for defining embryonic dorsoventral polarity. Finally, certain combinations ofnudelalleles simultaneously produce completely dorsalized and normal embryos yet very few embryos of intermediate mutant phenotypes. The unusual biphasic distribution of phenotypes may indicate thatnudelactivity above a threshold is required to generate embryonic dorsoventral polarity.