Niche differentiation of denitrifying anaerobic methane oxidizing bacteria and archaea leads to effective methane filtration in a Tibetan alpine wetland.
Niche differentiation of denitrifying anaerobic methane oxidizing bacteria and archaea leads to effective methane filtration in a Tibetan alpine wetland.
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DOI：
10.1016/j.envint.2020.105764
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发表时间：
2020-05
影响因子：
11.8
通讯作者：
Fei Xie;Anzhou Ma;Hanchang Zhou;Yu Liang;Jun Yin;Kendall Ma;X. Zhuang;G. Zhuang
中科院分区：
文献类型：
--
作者：
Fei Xie;Anzhou Ma;Hanchang Zhou;Yu Liang;Jun Yin;Kendall Ma;X. Zhuang;G. Zhuang
反硝化厌氧甲烷氧化(达摩)是湿地中重要的甲烷汇。然而，达摩细菌和古细菌在淡水湿地土壤中的相互作用和生态位分配，以及耦合甲烷和氮循环的微生物之间的相互作用仍不清楚，尽管这些因素可能决定湿地中甲烷和氮素的命运。在这里，我们评估了达摩细菌和古菌在西藏淡水湿地甲烷耦合氮循环微生物群落中的垂直分布以及种群之间的潜在相互作用。结合分子生物学、稳定同位素示踪技术和微生物生物信息学来评估这些相互关联的动力学。甲烷的丰度和潜在氧化速率表明，达摩细菌和古细菌分别占据表层和亚表层土壤。达摩细菌和氮循环微生物在其群落内的相互作用是复杂的，达摩细菌显然在竞争激烈的表层土壤环境中获得了优势，并在这些环境中占据了特定的生态位。相反，达摩古生菌与氮循环微生物之间的表观关系相对简单，推测达摩古生菌与地下土层中的硝酸盐产生菌之间存在高度的合作。这些结果表明，达摩细菌和古细菌的垂直分布格局使它们能够在不同土层的甲烷氧化活性中发挥重要作用，共同形成一个有效的甲烷过滤联合体。
Denitrifying anaerobic methane oxidation (DAMO) is a vital methane sink in wetlands. However, the interactions and niche partitioning of DAMO bacteria and archaea in freshwater wetland soils, in addition to the interactions among microorganisms that couple methane and nitrogen cycling is still unclear, despite that these factors may govern the fate of methane and nitrogen in wetlands. Here, we evaluated the vertical distribution of DAMO bacteria and archaea in soil layers along with the potential interactions among populations in the methane-coupled nitrogen cycling microbial community of Tibetan freshwater wetlands. A combination of molecular biology, stable isotope tracer technology, and microbial bioinformatics was used to evaluate these interrelated dynamics. The abundances and potential methane oxidation rates indicated that DAMO bacteria and archaea differentially occupy surface and subsurface soil layers, respectively. The inferred interactions between DAMO bacteria and nitrogen cycling microorganisms within their communities are complex, DAMO bacteria apparently achieve an advantage in the highly competitive environment of surface soils layers and occupy a specific niche in those environments. Conversely, the apparent relationships between DAMO archaea and nitrogen cycling microorganisms are relatively simple, wherein high levels of cooperation are inferred between DAMO archaea and nitrate-producing organisms in subsurface soils layers. These results suggest that the vertical distribution patterns of DAMO bacteria and archaea enable them to play significant roles in the methane oxidation activity of different soil layers and collectively form an effective methane filtration consortium.