Effects of cardiac patches engineered with bone marrow-derived mononuclear cells and PGCL scaffolds in a rat myocardial infarction model
Effects of cardiac patches engineered with bone marrow-derived mononuclear cells and PGCL scaffolds in a rat myocardial infarction model
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DOI：
10.1016/j.biomaterials.2006.09.009
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Cho, Myeong-Chan
中科院分区：
文献类型：
--
作者：
Piao, Hainan;Kwon, Jin-Sook;Cho, Myeong-Chan
目前对心衰（HF）的心脏保护作用、对骨髓源性单核细胞（BMMNC）分化的影响以及BMMNC种子的可生物降解聚乙二醇-co-己内酯（PGCL）支架在心肌梗死（MI）动物模型中的生物相容性知之甚少。本研究假设将BMMNC种子PGCL支架植入心肌梗死模型大鼠心外膜表面具有生物相容性，可诱导BMMNC向梗死心肌迁移，有效改善左心室收缩功能障碍。植入bmmnc种子PGCL支架一周后，BMMMC向心外膜区域迁移。植入后4周，梗死区及梗死边界区新生血管增强。一些bmmnc表现出α - mhc和肌钙蛋白I的存在，这是向心肌细胞分化的标志。在超声心动图检查中，与对照组相比，bmmnc植入的PGCL支架组和非细胞植入的单纯PGCL支架组有效地降低了左室进行性扩张，并保留了左室收缩功能。因此，BMMNC种子PGCL支架影响BMMNC迁移、向心肌细胞分化和诱导新生血管，最终有效减轻左室重塑和进行性左室收缩功能障碍。PGCL支架可以被认为是mi诱导的晚期HF的有效治疗选择。(c) 2006 Elsevier Ltd.版权所有。
Little is known about the cardioprotective effects against heart failure (HF), the effects on differentiation of bone marrow-derived mononuclear cell (BMMNC), and the biocompatibility of BMMNC-seeded biodegradable poly-glycolide-co-caprolactone (PGCL) scaffolds in a myocardial infarction (MI) animal model. This study hypothesized that implantation of a BMMNC-seeded PGCL scaffold into the epicardial surface in a rat MI model would be biocompatible, induce BMMNC migration into infarcted myocardium, and effectively improve left ventricular (LV) systolic dysfunction. One week after the implantation of a BMMNC-seeded PGCL scaffold, BMMMC showed migration into the epicardial region. Four weeks after implantation, augmented neovascularization was observed in infarcted areas and in infarct border zones. Some BMMNCs exhibited the presence of alpha-MHC and troponin I, markers of differentiation into cardiomyocytes. In echocardiographic examinations, BMMNC-seeded PGCL scaffold and non-cell-seeded simple PGCL scaffold groups effectively reduced progressive LV dilatation and preserved LV systolic function as compared to control rat MI groups. Thus, BMMNC-seeded PGCL scaffolding influences BMMNC migration, differentiation to cardiomyocytes, and induction of neovascularization, ultimately effectively lessening LV remodeling and progressive LV systolic dysfunction. PGCL scaffolding can be considered as an effective treatment alternative in MI-induced advanced HF. (c) 2006 Elsevier Ltd. All rights reserved.