The presence of a new 3-oxoacyl-CoA thiolase in rat liver peroxisomes.
The presence of a new 3-oxoacyl-CoA thiolase in rat liver peroxisomes.
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大鼠肝脏过氧化物酶体中存在新的 3-氧代酰基-CoA 硫解酶。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
T. Hashimoto
中科院分区：
文献类型：
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作者：
S. Miyazawa;T. Osumi;T. Hashimoto
在大鼠肝脏中发现了一种新型 3-草酰辅酶 A 硫解酶。通过给大鼠施用邻苯二甲酸二(2-乙基己基)酯来诱导这些活性，从而增强过氧化物酶体β-氧化活性，然后从大鼠肝脏中纯化了这种硫解酶、线粒体通用3-草酰基-CoA硫解酶和乙酰乙酰-CoA硫解酶。新的 3-氧代酰基-CoA 硫解酶与线粒体和细胞质硫解酶的区别如下：DEAE-纤维素色谱、磷酸纤维素色谱、免疫化学滴定和底物特异性。肝脏的亚细胞分级分离和线粒体轻组分的蔗糖密度梯度离心表明，新的硫解酶主要存在于过氧化物酶体中。
A novel 3-oxacyl-CoA thiolase was found in rat liver. This thiolase, mitochondrial general 3-oxacyl-CoA thiolase and acetoacetyl-CoA thiolase were purified from the rat liver after the induction of these activities by the administration to rats of di(2-ethylhexyl)phthalate, which enhanced the peroxisomal beta-oxidation activity. The new 3-oxoacyl-CoA thiolase was distinguished from mitochondrial and cytoplasmic thiolases by the following: DEAE-cellulose chromatography, phosphocellulose chromatography, immunochemical titration, and substrate specificity. Subcellular fractionation of liver and sucrose-density-gradient centrifugation of the light mitochondrial fraction revealed that the new thiolase was mainly in peroxisomes.