LUNG DEFENSES AGAINST PSEUDOMONAS-AERUGINOSA IN C5-DEFICIENT MICE WITH DIFFERENT GENETIC BACKGROUNDS
LUNG DEFENSES AGAINST PSEUDOMONAS-AERUGINOSA IN C5-DEFICIENT MICE WITH DIFFERENT GENETIC BACKGROUNDS
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DOI：
10.1128/iai.52.3.853-857.1986
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发表时间：
1986-06-01
影响因子：
3.1
通讯作者：
HOOKE, AM
中科院分区：
文献类型：
--
作者：
CERQUETTI, MC;SORDELLI, DO;HOOKE, AM
在具有不同遗传背景的C5缺陷型小鼠品系中研究肺对铜绿假单胞菌的防御。我们研究了C5缺陷B10.D2/oSnJ和DBA/2 J小鼠及其最接近的C5充足对应物B10.D2/nSnJ和DBA/1 J小鼠气雾剂暴露于铜绿假单胞菌后的肺清除率和细胞反应。两种C5缺陷型菌株的肺清除率和肺细胞反应模式不同。与C5充足的B10.D2/nSnJ动物相比，C5缺乏的B10.D2/oSnJ小鼠在激发后4小时显示铜绿假单胞菌的肺清除缺陷。这一发现与同期招募到气道中的多形核白细胞数量减少有关。有趣的是，C5缺乏的DBA/2 J小鼠招募更多的多形核白细胞比C5充足的DBA/1 J小鼠雾化后4小时。然而，DBA/2 J小鼠中铜绿假单胞菌的肺清除不如C5充足的DBA/1 J小鼠有效，这表明除了趋化作用之外，还可能涉及C5的其他功能。还在具有相同遗传背景的C5-缺陷和C5-充足的杂交体（DBA/2 JX）中研究了铜绿假单胞菌的肺清除。B10.D2）。结果表明，在特定的遗传背景下（B10.D2），小鼠肺对铜绿假单胞菌的清除率明显受到C5缺乏的影响。
Lung defenses against Pseudomonas aeruginosa were investigated in C5-deficient strains of mice with different genetic backgrounds. We studied pulmonary clearance and cell responses after aerosol exposure to P. aeruginosa in C5-deficient B10.D2/oSnJ and DBA/2J mice and their closest C5-sufficient counterparts, B10.D2/nSnJ and DBA/1J mice. Different patterns of lung clearance and pulmonary cell responses were found for the two C5-deficient strains. C5-deficient B10.D2/oSnJ mice showed defective lung clearance of P. aeruginosa 4 h after challenge compared with C5-sufficient B10.D2/nSnJ animals. This finding was associated with a decreased number of polymorphonuclear leukocytes recruited into the airways during the same time. Interestingly, C5-deficient DBA/2J mice recruited higher numbers of polymorphonuclear leukocytes than did C5-sufficient DBA/1J mice by 4 h after aerosolization. Nevertheless, lung clearance of P. aeruginosa in DBA/2J mice was not as effective as in C5-sufficient DBA/1J mice, suggesting that other functions of C5 besides chemotaxism could be involved. Lung clearance of P. aeruginosa was also investigated in C5-deficient and -sufficient hybrids sharing the same genetic background (DBA/2J .times. B10.D2). The results suggested that murine lung clearance of P. aeruginosa is markedly affected by lack of C5 in a specific genetic background (B10.D2).