Constitutive binding of yeast heat shock factor to DNA in vivo
Constitutive binding of yeast heat shock factor to DNA in vivo
复制标题
酵母热休克因子在体内与 DNA 的组成型结合
DOI：
10.1128/mcb.8.11.5040-5042.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Hugh R. B. Pelham
中科院分区：
文献类型：
--
作者：
B. K. Jakobsen;Hugh R. B. Pelham
我们通过干扰实验测量了酵母热休克因子（HSF）在体内与DNA的结合，其中HSF将GAL4从含有重叠结合位点的合成启动子元件中排除。结果表明，HSF在非应激细胞中与DNA结合，但这种结合不足以激活转录。
We measured the binding of yeast heat shock factor (HSF) to DNA in vivo by using an interference assay in which HSF excludes GAL4 from a synthetic promoter element containing overlapping binding sites for each protein. The results show that HSF binds to DNA in unstressed cells and that binding is not sufficient for transcriptional activation.