INTERACTION BETWEEN THE POLLEN-TUBE AND STIGMATIC CELL-WALL FOLLOWING POLLINATION IN BRASSICA-OLERACEA
INTERACTION BETWEEN THE POLLEN-TUBE AND STIGMATIC CELL-WALL FOLLOWING POLLINATION IN BRASSICA-OLERACEA
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DOI：
10.1111/j.1469-8137.1988.tb00225.x
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发表时间：
1988-05-01
期刊：
影响因子：
9.4
通讯作者：
DICKINSON, HG
中科院分区：
文献类型：
--
作者：
ELLEMAN, CJ;WILLSON, CE;DICKINSON, HG
电镜观察显示，甘蓝的柱头乳头壁由若干层组成。对细胞壁主要成分厚度的测量表明，两种S（不亲和性）基因型在细胞壁厚度上存在显著差异。柱头表面花粉水化率的差异与角质层厚度有关。讨论了这一发现与伪相容性遗传控制的相关性。不同寻常的是，乳突细胞壁的胸纤维素成分由三层组成。授粉时，外层脱落，使细长的花粉管沿着壁内的乳突向下传递。在乳头底部，管变成真正的细胞外，向下生长在花柱薄壁细胞之间的细胞间隙中。在花蕾授粉之后，花粉管甚至可以穿透整个细胞壁，沿着细胞的长度向下生长，一边是质膜，另一边是胸纤维素壁的内面。在进入花柱的细胞间隙之前，这些管在穿过乳突基部的细胞壁时遇到了一些困难。
Electron microscopic observations have revealed the stigmatic papilla wall of Brassica oleracea L. to be composed of several distinct layers. Measurement of the thickness of the major components of this wall has demonstrated a significant difference in cutcle thickness between the two S (incompatibility) genotypes under study. It is suggested that cuticular thickness is responsible for differences observed in the rates of pollen hydration on the stigma surface. The relevance of this finding to the genetical control of pseudocompatibility is discussed. Unusually, the pectocellulosic component of the papillar cell wall is composed of three layers. On pollination, the outer layer detaches to permit the elongating pollen tube to pass down the papilla within the wall itself. At the base of the papilla, the tube becomes truly extracellular, growing down in the intercellular spaces between stylar parenchyma cells. Following a bud pollination, the pollen tube may even penetrate the complete cell wall and grow down the length of the cell, invested on one side by the plasma membrane and on the other by the inner face of the pectocellulosic wall. These tubes experience some difficulty in traversing the cell wall at the base of the papillar prior to entering the intercellular spaces of the style.