Unusual Activity of a Chlamydomonas TET/JBP Family Enzyme.
Unusual Activity of a Chlamydomonas TET/JBP Family Enzyme.
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DOI：
10.1021/acs.biochem.9b00609
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发表时间：
2019-08
期刊：
影响因子：
2.9
通讯作者：
L. Aravind;S. Balasubramanian;A. Rao
中科院分区：
文献类型：
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作者：
L. Aravind;S. Balasubramanian;A. Rao
大约24.5亿年前发生在地球上的大氧化事件为酶开辟了一个生态位，这些酶可以利用分子氧（o2）直接氧化底物，包括2-氧戊二酸酯（2OG）和依赖铁（II）的双加氧酶。2ogfe -双加氧酶具有双链β -螺旋结构，铁中心由两个组氨酸和一个天冬氨酸螯合。2ogfe -双加氧酶催化一个o2原子与2-氧戊二酸盐结合，氧化成琥珀酸盐，第二个原子生成有机底物。AlkB家族和相关分支在碱基氮上的烷基加合物上催化该反应，而TET/JBP家族催化与碱基碳上连接的甲基氧化。作为通过修饰DNA或RNA产生表观遗传标记的酶，AlkB和TET/JBP家族的成员已经被反复招募到不同的真核生物谱系中。
The Great Oxygenation Event, which occurred on earth around 2.45 billion years ago, opened up the niche for enzymes that could directly oxidize substrates using molecular oxygen (O 2 ), including the 2-oxoglutarate (2OG) and Fe(II)-ependent dioxygenases. 2OGFe-dioxygenases possess a double-stranded β -helix, with an iron center chelated by two histidines and an aspartate. 1 2OGFe-dioxygenases catalyze the incorporation of one atom of O 2 into 2-oxoglutarate, oxidizing it to succinate, and the second atom into an organic substrate. The AlkB family and related clades catalyze this reaction on alkyl adducts on nitrogens of bases, whereas the TET/JBP family catalyzes the oxidation of methyl groups attached to carbons of bases. 1 As enzymes that generate epigenetic marks by modifying DNA or RNA, members of both the AlkB and TET/JBP families have been repeatedly recruited to different eukaryotic lineages.