CHARACTERIZATION OF THE GENOME OF PSEUDOMONAS-AERUGINOSA BACTERIOPHAGE-PHI-PLS27 WITH PARTICULAR REFERENCE TO THE ENDS OF THE DNA
CHARACTERIZATION OF THE GENOME OF PSEUDOMONAS-AERUGINOSA BACTERIOPHAGE-PHI-PLS27 WITH PARTICULAR REFERENCE TO THE ENDS OF THE DNA
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DOI：
10.1128/jvi.63.4.1587-1594.1989
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发表时间：
1989-04-01
影响因子：
5.4
通讯作者：
KROPINSKI, AM
中科院分区：
文献类型：
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作者：
ALLAN, BJ;DAVIES, P;KROPINSKI, AM
研究了铜绿假单胞菌粗特异性噬菌体φ PLS 27的DNA。通过将限制性片段的大小相加确定的基因组大小为42.7个内切酶对。特别令人感兴趣的是，DNA对某些常见的限制性内切酶，包括EcoRI、BamHI和HindIII不敏感。对噬菌体DNA的末端进行克隆和测序，揭示了318个核苷酸的直接重复序列。当克隆到启动子选择载体pKK 232 -8中时，基因组的左端在大肠杆菌中表现出启动子活性。鉴定了与质粒pNM 74 TOL操纵子和PAK菌毛蛋白启动子具有> 70%序列同源性的两个启动子。
The DNA of Pseudomonas aeruginosa rough-specific bacteriophage .phi.PLS27 was studied. The genome size as determined by summing the sizes of restriction fragments was 42.7 kilobase pairs. Of particular interest was the fact that the DNA was insensitive to certain common restriction endonucleases including EcoRI, BamHI, and HindIII. The ends of the phage DNA were cloned and sequenced, revealing direct repeats of 318 nucleotides. The left end of the genome when cloned into the promoter selection vector pKK232-8 exhibited promoter activity in Escherichia coli. Two promoters bearing > 70% sequence homology to the plasmid pNM74 TOL operon and PAK pilin promoters were identified.