Disturbance of the bacterial cell wall specifically interferes with biofilm formation
Disturbance of the bacterial cell wall specifically interferes with biofilm formation
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DOI：
10.1111/1758-2229.12346
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发表时间：
2015-12-01
影响因子：
3.3
通讯作者：
Kolodkin-Gal, Ilana
中科院分区：
文献类型：
--
作者：
Bucher, Tabitha;Oppenheimer-Shaanan, Yaara;Kolodkin-Gal, Ilana
在自然界中，细菌通过化学信号进行交流，并建立称为生物膜的复杂群落，其中细胞通过细胞外基质保持在一起。许多研究都集中在通过改变细胞信号通路来操纵和阻止生物膜组装的小分子上。然而，细菌细胞被膜，呈现细菌细胞与其周围环境之间的界面，在很大程度上被忽视。在我们的研究中，我们确定了不同细胞壁组分（肽聚糖，壁磷壁酸和磷壁酸）的生物合成途径中的特定靶标，这些组分阻碍生物膜形成和细胞外基质的锚定，对细胞外生长的影响最小。此外，我们提供了令人信服的证据表明，生物膜阻碍转糖基化抑制剂和D-亮氨酸触发高度特异性的反应，而不改变生物膜细胞内的整体蛋白质水平或细胞外基质成分的整体水平。所呈现的结果强调了革兰氏阳性细胞壁在生物膜发育、抗性和维持中的核心作用。
In nature, bacteria communicate via chemical cues and establish complex communities referred to as biofilms, wherein cells are held together by an extracellular matrix. Much research is focusing on small molecules that manipulate and prevent biofilm assembly by modifying cellular signalling pathways. However, the bacterial cell envelope, presenting the interface between bacterial cells and their surroundings, is largely overlooked. In our study, we identified specific targets within the biosynthesis pathways of the different cell wall components (peptidoglycan, wall teichoic acids and teichuronic acids) hampering biofilm formation and the anchoring of the extracellular matrix with a minimal effect on planktonic growth. In addition, we provide convincing evidence that biofilm hampering by transglycosylation inhibitors and D-Leucine triggers a highly specific response without changing the overall protein levels within the biofilm cells or the overall levels of the extracellular matrix components. The presented results emphasize the central role of the Gram-positive cell wall in biofilm development, resistance and sustainment.