Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery.
Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery.
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DOI：
10.1016/j.bbrc.2008.03.127
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发表时间：
2008-06
影响因子：
3.1
通讯作者：
Zhi-wen Zhang;X. Sha;Anle Shen;Yongzhong Wang;Zhaogui Sun;Zheng Gu;Xiaoling Fang
中科院分区：
文献类型：
--
作者：
Zhi-wen Zhang;X. Sha;Anle Shen;Yongzhong Wang;Zhaogui Sun;Zheng Gu;Xiaoling Fang
采用十六烷基聚乙烯亚胺（PEI）修饰纳米结构脂质载体（NLC），构建了一种新型的非病毒基因转移载体。采用乳化-溶剂挥发法制备聚阳离子纳米结构脂质载体。在人肺腺癌细胞系SPC-A1和中国人卵巢癌细胞（CHO）中评价其体外基因转移特性。在向PNLC制剂中添加三油酸甘油酯后观察到PNLC的转染效率增强，并且优化的PNLC的转染效率与Lipofectamine™2000的转染效率相当。在10%血清存在下，最佳PNLC的转染效率在任一细胞系中均未显著改变，而Lipofectamine™2000的转染效率在两者中均大大降低。因此，PNLC是一种有效的非病毒基因转移载体，并且在PNLC制剂中加入三油精后，PNLC的基因递送活性得到增强。
A novel nonviral gene transfer vector was developed by modifying nanostructured lipid carrier (NLC) with cetylated polyethylenimine (PEI). Polycation nanostructured lipid carrier (PNLC) was prepared using the emulsion–solvent evaporation method. Its in vitro gene transfer properties were evaluated in the human lung adenocarcinoma cell line SPC-A1 and Chinese Hamster Ovary (CHO) cells. Enhanced transfection efficiency of PNLC was observed after the addition of triolein to the PNLC formulation and the transfection efficiency of the optimized PNLC was comparable to that of Lipofectamine™2000. In the presence of 10% serum the transfection efficiency of the optimal PNLC was not significantly changed in either cell line, whereas that of Lipofectamine™2000 was greatly decreased in both. Thus, PNLC is an effective nonviral gene transfer vector and the gene delivery activity of PNLC was enhanced after triolein was included into the PNLC formulation.