Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses.
Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses.
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分支 DNA 扩增多聚体，用于灵敏、直接检测人类肝炎病毒。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Chu
中科院分区：
文献类型：
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作者：
M. Urdea;T. Horn;Fultz Tj;M. Anderson;Running Ja;Hamren Sj;D. Ahle;Chu
分支寡核苷酸（bDNA）已被合成，含有一个独特的主要部分和一组相同的二级片段共价连接到主要序列通过分支点。一级序列被设计为分别与靶核酸，例如B型肝炎病毒（HBV）或丙型肝炎病毒（HCV）基因组DNA或RNA杂交（直接或间接）。二级片段用于指导碱性磷酸酶标记的小寡核苷酸的多个拷贝的结合。已经设计了基于这些分支扩增多聚体的应用的HBV和HCV存在的测定。可以直接检测到少至1，000个肝炎病毒基因组。
Branched oligonucleotides (bDNA) have been synthesized containing a unique primary segment and a set of identical secondary fragments covalently attached to the primary sequence through branch points. The primary sequence is designed to hybridize (directly or indirectly) to a target nucleic acid, such as hepatitis B virus (HBV) or hepatitis C virus (HCV) genomic DNA or RNA, respectively. The secondary fragments are used to direct the binding of multiple copies of a small oligonucleotide labelled with alkaline phosphatase. Assays for the presence of HBV and HCV based on the application of these branched amplification multimers have been devised. It is possible to detect as few as 1,000 hepatitis viral genomes directly.