A spectrophotometric assay for dehydroascorbate reductase.
A spectrophotometric assay for dehydroascorbate reductase.
复制标题
脱氢抗坏血酸还原酶的分光光度测定。
DOI：
10.1016/0003-2697(83)90180-x
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Silber,R
中科院分区：
文献类型：
--
作者：
Stahl,RL;Liebes,LF;Farber,CM;Silber,R
描述了基于与抗坏血酸形成相关的吸光度变化的脱氢抗坏血酸还原酶的简单分光光度测定。使用从菠菜叶中提取的部分纯化的制剂，发现反应与时间和酶浓度呈线性关系。通过该测定测定的反应速率与通过高效液相色谱法获得的反应速率良好相关。讨论了相对于当前可用测定的可能优势以及潜在应用。
A simple spectrophotometric assay for dehydroascorbate reductase based on the change in absorbance associated with the formation of ascorbic acid is described. Using a partially purified preparation from spinach leaves, the reaction was found to be linear with time and enzyme concentration. The reaction rate determined by this assay correlated well with that obtained by a high-performance liquid chromatography method. Possible advantages over currently available assays as well as potential applications are discussed.