Multispectral imaging of tissue absorption and scattering using spatial frequency domain imaging and a computed-tomography imaging spectrometer
Multispectral imaging of tissue absorption and scattering using spatial frequency domain imaging and a computed-tomography imaging spectrometer
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DOI：
10.1117/1.3528628
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发表时间：
2011-01-01
影响因子：
3.5
通讯作者：
Tromberg, Bruce J.
中科院分区：
文献类型：
--
作者：
Weber, Jessie R.;Cuccia, David J.;Tromberg, Bruce J.
我们提出了一种方法，快速，定量映射组织的吸收和散射光谱在宽视场，非接触式成像几何结构相结合的多频空间频域成像（SFDI）与计算机断层扫描成像光谱仪（CTIS）。SFDI克服了空间扫描源的需要，并且基于周期性结构化照明图案的投影和分析。CTIS通过将多个光谱图像同时衍射到单个CCD芯片上来收集图像每个像素的光谱，从而在单个快照中提供空间和光谱信息，从而提供吞吐量优势。空间光谱数据集的采集速度比我们的波长扫描液晶可调滤光相机快30倍，即使它还没有优化速度。在这里，我们证明了结合SFDI-CTIS能够在非接触，非扫描平台的组织吸收和散射的快速，多光谱成像。组合系统在组织模拟体模中针对650-1000 nm之间的36个波长在组织样吸收和散射特性的范围内进行了验证。在650-800 nm范围内，吸收系数（μ（a））的平均百分比误差小于10%，在800-1000 nm范围内小于20%。约化散射系数（μ '（s））的平均百分比误差在650-700 nm范围内小于5%，在700-1000 nm范围内小于3%。将SFDI-CTIS平台应用于小鼠脑损伤模型，以证明这种方法在表征空间和光谱变化的组织光学特性方面的实用性。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI电话：10.1117/1.3528628
We present an approach for rapidly and quantitatively mapping tissue absorption and scattering spectra in a wide-field, noncontact imaging geometry by combining multifrequency spatial frequency domain imaging (SFDI) with a computed-tomography imaging spectrometer (CTIS). SFDI overcomes the need to spatially scan a source, and is based on the projection and analysis of periodic structured illumination patterns. CTIS provides a throughput advantage by simultaneously diffracting multiple spectral images onto a single CCD chip to gather spectra at every pixel of the image, thus providing spatial and spectral information in a single snapshot. The spatial-spectral data set was acquired 30 times faster than with our wavelength-scanning liquid crystal tunable filter camera, even though it is not yet optimized for speed. Here we demonstrate that the combined SFDI-CTIS is capable of rapid, multispectral imaging of tissue absorption and scattering in a noncontact, nonscanning platform. The combined system was validated for 36 wavelengths between 650-1000 nm in tissue simulating phantoms over a range of tissue-like absorption and scattering properties. The average percent error for the range of absorption coefficients (mu(a)) was less than 10% from 650-800 nm, and less than 20% from 800-1000 nm. The average percent error in reduced scattering coefficients (mu'(s)) was less than 5% from 650-700 nm and less than 3% from 700-1000 nm. The SFDI-CTIS platform was applied to a mouse model of brain injury in order to demonstrate the utility of this approach in characterizing spatially and spectrally varying tissue optical properties. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3528628]