Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the ribosomal protein gene cluster.
Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the ribosomal protein gene cluster.
复制标题
枯草芽孢杆菌 RNA 聚合酶 α 亚基的基因位于核糖体蛋白基因簇中。
DOI：
10.1128/jb.168.1.65-71.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Price,CW
中科院分区：
文献类型：
--
作者：
Suh,JW;Boylan,SA;Price,CW
我们使用抗α抗体作为探针，从lambda gt11表达载体库中分离出编码枯草芽孢杆菌RNA聚合酶α亚基的基因。分离出四个独特的克隆，一个携带 lacZ-α 基因融合体，三个携带整个 α 编码区以及上游的附加序列。通过其在大肠杆菌中表达的产物的大小和免疫反应性证实了克隆的α基因的身份。此外，部分DNA序列发现预测的α的NH2末端与大肠杆菌α同源。通过质粒整合和PBS1转导，我们将α定位在rpsE附近和枯草芽孢杆菌染色体上主要核糖体蛋白基因簇内。额外的 DNA 测序鉴定出 α 上游的 rpsM（编码 S13）和 rpsK（编码 S11），随后是可能包含两个 α 启动子的 180 个碱基对顺反子间区域。尽管 α 区域的组织类似于大肠杆菌的 α 操纵子，但紧邻枯草芽孢杆菌 α 基因之前的推定启动子和 rpsD（编码 S4）的缺失表明了不同的调节。
We isolated the gene encoding the alpha subunit of Bacillus subtilis RNA polymerase from a lambda gt11 expression vector library by using anti-alpha antibody as a probe. Four unique clones were isolated, one carrying a lacZ-alpha gene fusion and three carrying the entire alpha coding region together with additional sequences upstream. The identity of the cloned alpha gene was confirmed by the size and immunological reactivity of its product expressed in Escherichia coli. Further, a partial DNA sequence found the predicted NH2 terminus of alpha homologous with E. coli alpha. By plasmid integration and PBS1 transduction, we mapped alpha near rpsE and within the major ribosomal protein gene cluster on the B. subtilis chromosome. Additional DNA sequencing identified rpsM (encoding S13) and rpsK (encoding S11) upstream of alpha, followed by a 180-base-pair intercistronic region that may contain two alpha promoters. Although the organization of the alpha region resembles that of the alpha operon of E. coli, the putative promoters and absence of rpsD (encoding S4) immediately preceding the B. subtilis alpha gene suggest a different regulation.