On farm evaluation of the coproantigen ELISA and coproantigen reduction test in Scottish sheep naturally infected with Fasciola hepatica
On farm evaluation of the coproantigen ELISA and coproantigen reduction test in Scottish sheep naturally infected with Fasciola hepatica
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DOI：
10.1016/j.vetpar.2012.02.009
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发表时间：
2012-07-06
影响因子：
2.6
通讯作者：
Skuce, P. J.
中科院分区：
文献类型：
--
作者：
Gordon, D. K.;Zadoks, R. N.;Skuce, P. J.
肝吸虫肝片吸虫是绵羊养殖中造成重大经济损失的一个原因。在活体动物中缺乏方便和敏感的诊断试验，阻碍了监测感染状况和治疗效果的能力。使用coproantigen ELISA和基于该ELISA的coproantigen还原试验可以解决这些问题，但迄今为止，仅在实验性激发研究中进行了评估。我们在苏格兰农场的工作条件下对粪原抗原ELISA进行了评估，以评估其作为肝吸虫感染诊断测试的有效性，并作为诊断测试来帮助确定杀虫剂对绵羊的治疗效果。首先，对一组羔羊的肝吸虫感染状况进行纵向监测，使用每月的血液样本进行生化检测和血清抗体ELISA，使用每月的粪便样本进行粪卵计数（FEC）和粪原抗原ELISA。平均血清抗体ELISA滴度在9月呈阳性，比粪便中吸虫感染的指标早两个月。与实验挑战研究的结果相反。FEC和coproantigen ELISA在同一时间点呈阳性。其次，对来自两个农场的100只母羊进行了治疗效果的测量，分别用三氯苯达唑（TCBZ）和克桑特（closantel）治疗。治疗后7、14和21天的粪卵计数减少试验和粪原抗原减少试验在组水平上的治疗效果估计值相似。对于单个动物，观察到测试之间的一些不一致。两个养殖场均发现TCBZ治疗失败，尽管对动物进行了准确的称重和治疗产品的剂量。我们得出结论：(1)粪原抗原ELISA是一种比粪卵计数更方便的检测方法，有望作为绵羊自然吸虫感染的诊断工具，但可能需要进一步评估解释标准；(2)粪原抗原ELISA在现场的表现与绵羊的实验攻毒研究不同；(3)两个农场都存在抗tcbz吸虫。(C) 2012 Elsevier B.V.版权所有
The liver fluke, Fasciola hepatica, is a cause of significant economic losses in sheep farming. Lack of convenient and sensitive diagnostic tests in the live animal hampers the ability to monitor infection status and treatment efficacy. Use of a coproantigen ELISA and coproantigen reduction test, based on this ELISA, may address these issues but has, to date, only been evaluated in experimental challenge studies. We evaluated the coproantigen ELISA under working farm conditions in Scotland to assess its usefulness as a diagnostic test for liver fluke infection and as a diagnostic test to help determine the efficacy of flukicide treatment in sheep. First, liver fluke infection status was monitored longitudinally in a group of lambs, using monthly blood samples for biochemical assays and serum antibody ELISA and using monthly faecal samples for faecal egg count (FEC) and coproantigen ELISA. The average serum antibody ELISA titre became positive in September, two months ahead of faecal indicators of fluke infection. In contrast to results from experimental challenge studies. FEC and coproantigen ELISA became positive at the same time point. Secondly, treatment efficacy was measured in 100 ewes, from two farms, after treatment with triclabendazole (TCBZ) or closantel. Group level estimates of treatment efficacy were similar between faecal egg count reduction testing and coproantigen reduction testing at 7, 14 and 21 days post treatment. For individual animals, some inconsistencies between tests were observed. TCBZ treatment failure was noted on both farms, despite accurate weighing of animals and dosing of treatment products. We conclude that (1) coproantigen ELISA is a more convenient test than faecal egg counts and holds promise as a diagnostic tool for natural fluke infections in sheep but further evaluation of interpretation criteria may be needed: (2) the coproantigen ELISA has performed differently in the field compared with experimental challenge studies in sheep and (3) TCBZ-resistant fluke were present on both farms. (C) 2012 Elsevier B.V. All rights reserved.