Fragments of Target Cells are Internalized into Retroviral Envelope Protein-Expressing Cells during Cell-Cell Fusion by Endocytosis.
Fragments of Target Cells are Internalized into Retroviral Envelope Protein-Expressing Cells during Cell-Cell Fusion by Endocytosis.
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靶细胞的片段通过内吞作用在细胞 - 细胞融合过程中内部内部表达逆转录病毒包膜蛋白的细胞。
DOI：
10.3389/fmicb.2015.01552
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发表时间：
2015
影响因子：
5.2
通讯作者：
Kubo Y
中科院分区：
文献类型：
--
作者：
Izumida M;Kamiyama H;Suematsu T;Honda E;Koizumi Y;Yasui K;Hayashi H;Ariyoshi K;Kubo Y
逆转录病毒通过病毒和宿主细胞膜之间的融合进入宿主细胞。逆转录病毒包膜糖蛋白（Env）诱导膜融合，也介导细胞-细胞融合。存在两种类型的由Env蛋白诱导的细胞-细胞融合。自内融合是由病毒融合Env蛋白表达细胞和易感细胞之间的融合诱导的，而病毒粒子通过相邻细胞之间的融合诱导自外融合。虽然嗜亲性小鼠白血病病毒（E-MLV）的进入需要宿主细胞内吞作用，但内吞作用在细胞融合中的参与尚不清楚。通过荧光显微镜分析内融合，我们发现靶细胞的片段被内化到Env表达细胞中。用内吞作用抑制剂处理Env表达细胞比靶细胞更显著地抑制细胞融合，表明Env表达细胞中的内吞作用是细胞融合所必需的。内吞作用抑制剂也减弱了从没有融合。电子显微镜分析表明，导致融合从内开始在内吞膜凹陷的膜融合。本研究表明，两种类型的病毒细胞融合都需要内吞作用，并提供了级联的融合从内。
Retroviruses enter into host cells by fusion between viral and host cell membranes. Retroviral envelope glycoprotein (Env) induces the membrane fusion, and also mediates cell-cell fusion. There are two types of cell-cell fusions induced by the Env protein. Fusion-from-within is induced by fusion between viral fusogenic Env protein-expressing cells and susceptible cells, and virions induce fusion-from-without by fusion between adjacent cells. Although entry of ecotropic murine leukemia virus (E-MLV) requires host cell endocytosis, the involvement of endocytosis in cell fusion is unclear. By fluorescent microscopic analysis of the fusion-from-within, we found that fragments of target cells are internalized into Env-expressing cells. Treatment of the Env-expressing cells with an endocytosis inhibitor more significantly inhibited the cell fusion than that of the target cells, indicating that endocytosis in Env-expressing cells is required for the cell fusion. The endocytosis inhibitor also attenuated the fusion-from-without. Electron microscopic analysis suggested that the membrane fusion resulting in fusion-from-within initiates in endocytic membrane dents. This study shows that two types of the viral cell fusion both require endocytosis, and provides the cascade of fusion-from-within.
CD4+ T淋巴细胞中的人类免疫缺陷病毒（HIV）感染在LFA-1上缺乏LFA-1：LFA-1是HIV介导的细胞融合所必需的，但对于病毒传播不足。
影响因子：
15.3
作者：
Pantaleo, G;Butini, L;Graziosi, C;Poli, G;Schnittman, S M;Greenhouse, J J;Gallin, J I;Fauci, A S
通讯作者：
Fauci, A S