Molecular ring toss of circular BAC DNA using micropillar array for single-molecule studies
Molecular ring toss of circular BAC DNA using micropillar array for single-molecule studies
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DOI：
10.1063/1.5142666
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发表时间：
2020-01-01
期刊：
影响因子：
3.2
通讯作者：
Terao, Kyohei
中科院分区：
文献类型：
--
作者：
Dohi, Daiki;Hirano, Ken;Terao, Kyohei
本文报道了一种利用微柱阵列器件捕获环形DNA分子并以高空间分辨率成像其动力学的方法。在荧光显微镜下，我们成功地将环状细菌人工染色体DNA分子捕获在微通道层流中的微柱式“环抛”中，并演示了精胺溶液诱导的流动和凝聚过程对其延伸的成像。结果表明，DNA分子的动态变化是由周围化学环境的微流体控制引起的。这一方法有望有助于阐明环状DNA分子的物理特性和动力学。
This paper reports a method for trapping circular DNA molecules and imaging the dynamics with high spatial resolution using a micropillar-array device. We successfully trapped circular bacterial artificial chromosome DNA molecules at a micropillar-based "ring toss" in the laminar flow of a microchannel under a fluorescence microscope and demonstrated the imaging of their extension by flow and condensation process induced by spermine solution. DNA molecules were visualized in an extended loop conformation, allowing high spatial resolution, and the results showed that the dynamics is induced by the microfluidic control of the surrounding chemical environment. The method is expected to lead to the elucidation of the physical characteristics and the dynamics of circular DNA molecules.