BK channel blockers inhibit potassium-induced proliferation of human astrocytoma cells
BK channel blockers inhibit potassium-induced proliferation of human astrocytoma cells
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DOI：
10.1097/00001756-200203250-00008
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发表时间：
2002-03-25
期刊：
影响因子：
1.7
通讯作者：
Patt, S
中科院分区：
文献类型：
--
作者：
Basrai, D;Kraft, R;Patt, S
BK通道在胶质瘤细胞中一直表达，其功能作用尚不清楚。在这里，我们表明，BK通道是定期活跃在人类I32 INI星形细胞瘤细胞在生理膜电位。将5 mM生理外部[K+]下的细胞增殖与20 mM升高的外部[K+]下的细胞增殖进行比较，模拟体内快速生长的坏死肿瘤的情况。10-30 mM范围内的高细胞外[K+]显著增加I32 INI细胞的增殖。这种K+诱导的增殖可以通过应用特异性BK通道阻断剂伊比利亚毒素（IBTX）或ImM四乙基铵（TEA）完全消除。在5 mM [K+]下，两种阻滞剂对细胞生长均无任何影响（e）。这些发现表明BK通道在星形细胞瘤细胞增殖中的特殊作用。
The functional role of BK channels, which are consistently expressed in glioma cells, is not clear. Here we show that the BK channels are regularly active in human I32INI astrocytoma cells at physiological membrane potentials. The proliferation of the cells at the physiological external [K+] of 5 mM is compared with that at the elevated external [K+] of 20 mM, simulating the situation in rapidly growing, necrotic tumours in vivo. High extracellular [K+] in the range 10-30 mM significantly increases the proliferation of I32INI cells. This K+ induced proliferation can be completely abolished by applying the specific BK channel blockers iberiotoxin (IBTX) or I mM tetraethylammonium (TEA). Neither blocker has any effect on cell growth at 5 mM [K+](e). These findings indicate a particular role of BK channels in astrocytoma cell proliferation.