Quantitative analysis of platelet activating factor (AGEPC) by fast atom bombardment mass spectrometry
Quantitative analysis of platelet activating factor (AGEPC) by fast atom bombardment mass spectrometry
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快原子轰击质谱法定量分析血小板活化因子（AGEPC）
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
R. Murphy
中科院分区：
文献类型：
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作者：
K. Clay;D. O. Stene;R. Murphy
血小板活化因子最近在结构上被描述为磷脂1-O-烷基-2-O-乙酰基-sn-甘油磷酸胆碱。据报道，它是一种公认的免疫反应化学介质以及抗高血压药物。本文描述了一种以氘标记的1-O-烷基-2-O-乙酰基-sn-甘油磷酸胆碱为内标物定量1-O-烷基-2-O-乙酰基-sn-甘油磷酸胆碱的方法。当使用60 ng的2 H3标记化合物时，可以测量低至10 ng的量。该定量分析的标准曲线通常具有0.999的相关系数，即使当FAB的信号饱和效应明显时。因此，当采用稳定同位素内标法时，使用FAB电离技术进行定量分析是可行的。
Platelet activating factor has recently been structurally described as the phospholipid 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine. It has been reported to be a putative chemical mediator of immunological reactions as well as an antihypertensive agent. A method to quantitate 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine using deuterium labeled 1-O-alkyl-2-O-acetyl-sn-glycerophosphocholine as an internal standard is described. When 60 ng of the2H3 labeled compound is employed, quantities as low as 10 ng can be measured. The standard curve for this quantitative analysis typically had a correlation coefficient of 0.999 even when the signal saturation effect of FAB was apparent. Thus quantitative analysis using the FAB ionization technique is feasible when one employs the stable isotope internal standard method.
炎症和过敏的脂质介质。
DOI：
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发表时间：
1982
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
O'Flaherty,JT
通讯作者：
O'Flaherty,JT