THE EFFECT ON THE CHICK EMBRYO OF THE SIMULTANEOUS INOCULATION OF STOOL, STREPTOMYCIN, AND PENICILLIN
THE EFFECT ON THE CHICK EMBRYO OF THE SIMULTANEOUS INOCULATION OF STOOL, STREPTOMYCIN, AND PENICILLIN
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DOI：
10.1126/science.104.2707.460
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发表时间：
1946-01-01
期刊：
影响因子：
56.9
通讯作者：
HODGES, JH
中科院分区：
文献类型：
--
作者：
HODGES, JH
13、14日龄鸡胚经Burnet法羊膜内接种0.25 ~ 0.30 ml。鸡蛋被密封起来，在37度的温度下孵育。40个小时。获得三种不同的粪便标本：2种在干冰上放置1-3个月。1例采自流行性腹泻和呕吐患者，1例新鲜“正常”标本。对照组用无菌生理盐水代替粪便悬浮液。大约40小时后。每个卵无菌打开，胚胎观察，液体培养。几乎所有接受含生理盐水或培西林粪便悬浮液的卵子都发生胚胎死亡。这些鸡蛋的培养总是阳性的。在相应的对照中，约有2/3的胚胎存活，40个胚胎中只有3个培养阳性。一半的胚胎在接种了粪便悬浮液、链霉素和青霉素的卵子中存活。在相应的对照组中也是如此。用粪便悬浮液和链霉素接种后，约有1/3的胚胎存活。阳性培养的数量与对照组的污染率大致相同。
Thirteen-or 14-day old chick embryos were given 0.25-0.30 ml. of inoculum by the intra-amniotie method of Burnet. The eggs were sealed and incubated at 37 [degree]C. for 40 hrs. Three different stool specimens were obtained: 2 which were on dry ice for 1-3 mos., taken from patients with epidemic diarrhea and vomiting, and 1 fresh "normal" specimen. Controls contained sterile saline instead of stool suspension. After about 40 hrs. each egg was opened aseptically, the embryo observed, and fluid cultured. Death of embryos occurred in nearly all eggs which received stool suspension with saline or with petiicillin. Cultures from these eggs were always positive. In corresponding controls, about 2/3 of the embryos survived, and only 3 of 40 had positive cultures. Half the embryos survived in eggs inoculated with stool suspension, streptomycin, and penicillin. The same was true in corresponding controls. About 1/3 of the embryos survived when inoculated with stool suspension and streptomycin. The number of positive, cultures was about the same as the contamination rate in the controls.