Genome-Wide Identification of WRKY Transcription Factors in Chinese jujube (Ziziphus jujuba Mill.) and Their Involvement in Fruit Developing, Ripening, and Abiotic Stress
Genome-Wide Identification of WRKY Transcription Factors in Chinese jujube (Ziziphus jujuba Mill.) and Their Involvement in Fruit Developing, Ripening, and Abiotic Stress
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中国枣 (Ziziphus jujuba Mill.) WRKY 转录因子的全基因组鉴定及其在果实发育、成熟和非生物胁迫中的作用
DOI：
10.3390/genes10050360
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发表时间：
2019-05-01
期刊：
影响因子：
3.5
通讯作者：
Huang, Jian
中科院分区：
文献类型：
--
作者：
Chen, Xin;Chen, Ruihong;Huang, Jian
枣（Ziziphus dizoba Mill.）是我国重要的经济水果作物，主要在北方干旱高盐地区种植。WRKY转录因子（TFs）参与植物发育和对多种非生物胁迫的应答。在本研究中，我们在全基因组水平上鉴定了骏枣和冬枣的61个和52个ZjWRKY转录因子。组织表达谱分析表明，7个基因在骏枣的所有组织中均组成性高表达。转录组分析表明，骏枣果实成熟过程中有39个ZjWRKY基因表达。在这些基因中，有19个基因的转录本丰度在骏枣和青尖酸枣果实中存在差异表达。此外，RT-qPCR分析表明，30，14，和18 ZjWRKY基因响应干旱，NaCl和阿坝处理，分别。因此，研究ZjWRKY基因在枣果实发育、成熟过程中的表达动态及其在枣和酸枣中的差异，为深入研究ZjWRKY基因在枣果实成熟调控中的作用提供了理论依据。此外，这些ZjWRKY基因对干旱和盐胁迫反应强烈，这为促进耐盐育种提供了候选ZjWRKY基因。
Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit crop in China and mainly cultivated on land with high salinity and drought conditions in northern China. WRKY transcription factors (TFs) are involved in plant development and in responses to multiple abiotic stresses. In this study, we identified 61 and 52 putative ZjWRKY TFs in Junzao' and Dongzao' at the genome-wide level. Tissue expression profiling showed that 7 genes were constitutively expressed at high level in all tissues of Junzao'. Transcriptome analysis revealed that 39 ZjWRKY genes were expressed during Junzao' jujube fruit ripening. Among these genes, the transcript abundance of 19 genes were differentially expressed between Junzao' and Qingjiansuanzao' fruit. In addition, RT-qPCR analyses revealed that 30, 14, and 18 ZjWRKY genes responded to drought, NaCl, and ABA treatments, respectively. Taken together, ZjWRKY genes expression dynamics during jujube fruit development, ripening, and their differences between jujube and wild jujube would provide insights into their possible roles regulating fruit ripening. In addition, those ZjWRKY genes responded strongly to drought and salt stress, which provide candidate ZjWRKY genes for facilitating tolerance breeding.