Antibiotic-producing bacteria from stag beetle mycangia
Antibiotic-producing bacteria from stag beetle mycangia
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DOI：
10.5582/ddt.2015.01000
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发表时间：
2015-02-01
影响因子：
3.1
通讯作者：
Kaito, Chikara
中科院分区：
文献类型：
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作者：
Miyashita, Atsushi;Hirai, Yuuki;Kaito, Chikara
寻找新的抗生素或抗真菌剂对于感染性疾病的化疗至关重要。土壤细菌资源的有限性使其难以发现新的候选药物。因此，我们把重点放在另一种细菌资源，而不是土壤细菌，昆虫物种的微生物植物群。从河北锹甲、直锹甲和大锹甲的菌丝体中分离到40株细菌和真菌。我们确定了这些物种与他们的核糖体DNA序列，并透露，克雷伯氏菌属。是鹿角甲虫菌囊中最常见的共生体。我们检查了这些微生物是否产生针对革兰氏阴性细菌大肠杆菌、革兰氏阳性细菌金黄色葡萄球菌或真菌新型隐球菌的抗生素。33、29和18株菌株的培养上清对大肠杆菌有抗菌活性。coli、S. aureus或C. neoformans，分别。这些发现表明，鹿角甲虫菌囊中存在的细菌是筛选新型抗生素的有用资源。
The search for new antibiotics or antifungal agents is crucial for the chemotherapies of infectious diseases. The limited resource of soil bacteria makes it difficult to discover such new drug candidate. We, therefore, focused on another bacterial resource than soil bacteria, the microbial flora of insect species. In the present study, we isolated 40 strains of bacteria and fungi from the mycangia of three species of stag beetle, Dorcus hopei binodulosus, Dorcus rectus, and Dorcus titanus pilifer. We identified those species with their ribosomal DNA sequences, and revealed that Klebsiella spp. are the most frequent symbiont in the stag beetle mycangia. We examined whether these microorganisms produce antibiotics against a Gram-negative bacterium, Escherichia coli, a Gram-positive bacterium, Staphylococcus aureus, or a fungus, Cryptococcus neoformans. Culture supernatants from 33, 29, or 18 strains showed antimicrobial activity against E. coli, S. aureus, or C. neoformans, respectively. These findings suggest that bacteria present in the mycangia of stag beetles are useful resources for screening novel antibiotics.