Apoptosis induced in an early step of African swine fever virus entry into Vero cells does not require virus replication
Apoptosis induced in an early step of African swine fever virus entry into Vero cells does not require virus replication
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DOI：
10.1006/viro.2001.1348
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发表时间：
2002-03-15
期刊：
影响因子：
3.7
通讯作者：
Revilla, Y
中科院分区：
文献类型：
--
作者：
Carrascosa, AL;Bustos, MJ;Revilla, Y
非洲猪瘟病毒（ASFV）感染后，允许的Vero细胞发生凋亡，其特征为DNA片段化、半胱天冬酶激活、线粒体细胞色素c的胞质释放和DNA含量的流式细胞术分析。为了确定病毒复制中触发细胞凋亡的步骤，我们使用了紫外线灭活病毒、蛋白质和核酸合成抑制剂以及阻断病毒脱壳的亲溶酶体药物。ASFV诱导的细胞凋亡伴随着caspase-3的激活，即使在阿糖胞苷或放线菌酮存在下也能检测到，表明激活凋亡过程不需要病毒DNA复制和蛋白质合成。caspase-3的激活在病毒吸收后2 h从氯喹抑制中释放，而紫外线灭活的ASFV感染细胞后并不诱导caspase级联反应的激活，我们认为ASFV通过一条细胞内途径诱导细胞凋亡，这条途径可能是在病毒脱壳过程中触发的。(C)2002 Elsevier Science（美国）。
Permissive Vero cells develop apoptosis, as characterized by DNA fragmentation, caspases activation, cytosolic release of mitochondrial cytochrome c, and flow cytometric analysis of DNA content, upon infection with African swine fever virus (ASFV). To determine the step in virus replication that triggers apoptosis, we used UV-inactivated virus, inhibitors of protein and nucleic acid synthesis, and lysosomotropic drugs that block virus uncoating. ASFV-induced apoptosis was accompanied by caspase-3 activation, which was detected even in the presence of either cytosine arabinoside or cycloheximide, indicating that viral DNA replication and protein synthesis were not required to activate the apoptotic process The activation of caspase-3 was released from chloroquine inhibition 2 h after virus absorption, while the infection with UV-inactivated ASFV did not induce the activation of the caspase cascade, We conclude that ASFV induces apoptosis in the infected cell by an intracellular pathway probably triggered during the process of virus uncoating. (C) 2002 Elsevier Science (USA).