Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3
Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3
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DOI：
10.1016/j.jep.2009.02.022
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发表时间：
2009-05-04
影响因子：
5.4
通讯作者：
Guan, Hai-tao
中科院分区：
文献类型：
--
作者：
Dai, Zhi-jun;Gao, Jie;Guan, Hai-tao
研究目的：苦参碱是一种从中药苦参中纯化的生物碱，众所周知具有抗炎、抗纤维化和抗癌等活性。本研究探讨苦参碱诱导胃癌细胞凋亡的机制。材料与方法：采用MTT法检测SGC-7901细胞增殖情况。透射电镜下观察细胞形态。采用流式细胞仪（FCM）通过annexinV-FITC/PI染色观察SGC-7901细胞的凋亡情况。使用间接免疫荧光方法通过 FCM 分析监测 SGC-7901 细胞中 Fas/FasL 的表达水平。采用荧光分光光度法测定caspase-3酶活性。结果：MTT法显示苦参碱对SGC-7901细胞增殖的抑制作用呈剂量依赖性和时间依赖性。通过电镜下的形态变化和FCM分析证明细胞凋亡诱导。苦参碱处理后，Fas 和 FasL 的花色强度水平同样上调，且均与细胞凋亡率相关。苦参碱组caspase-3酶活性升高，与细胞凋亡率呈正相关。结论：苦参碱在体外能够抑制细胞增殖，诱导SGC-7901细胞凋亡。细胞凋亡诱导似乎是通过上调 Fas/FasL 表达并激活 caspase-3 酶来进行的。 (C) 2009 Elsevier Ireland Ltd. 保留所有权利。
Aim of the study: Matrine, an alkaloid purified from the chinese herb Sophora flavescens Ait, is well known to possess activities including anti-inflammation, anti-fibrotic and anticancer. In this study, the mechanism of matrine inducing the apoptosis of gastric carcinoma cells was investigated.Materials and Methods: Proliferation of SGC-7901 cells was examined by MTT assay. Cellular morphology was observed under transmission electron microscope. Flow cytometry (FCM) was used to observe the apoptosis of SGC-7901 cells by staining with annexinV-FITC/PI. The expression levels of Fas/FasL in SGC-7901 cells were monitored by FCM analysis using an indirect immunoflurenscence method. Activity of caspase-3 enzyme was measured by spectrofluorometry.Results: MTT assay showed that matrine inhibited SGC-7901 cells proliferation in a dose-dependent and time-dependent manner. Apoptosis induction was demonstrated by morphological changes under electron microscope and FCM analysis. Florescence intensity levels of Fas and FasL were found to be equally up-regulated after matrine treatment, which were both correlated with apoptosis rate. The activity of caspase-3 enzyme increased in matrine groups, positively correlated with apoptosis rate.Conclusions: Matrine could inhibit cell proliferation and induce apoptosis of SGC-7901 cells in vitro. The apoptosis induction appears to proceed by up-regulating Fas/FasL expression and activating caspase-3 enzyme. (C) 2009 Elsevier Ireland Ltd. All rights reserved.