Manual hematoxylin and eosin staining of mouse tissue sections.
Manual hematoxylin and eosin staining of mouse tissue sections.
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DOI：
10.1101/pdb.prot073411
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发表时间：
2014-06-02
影响因子：
--
通讯作者：
Munn, Robert J
中科院分区：
文献类型：
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作者：
Cardiff, Robert D;Miller, Claramae H;Munn, Robert J
苏木精和伊红（H&E）染色是用于对已固定、处理、包埋和切片的组织进行显微镜检查的标准。它可以手动或自动执行。出于经济原因，手动技术通常是低样品量设施的首选方法。本方案描述了固定、处理、石蜡包埋和切片的小鼠组织的手动H&E染色。在H& E染色的组织中，核酸染成深蓝色，蛋白质染成红色至粉红色或橙子。为了准确的表型和组织细节的描绘，必须严格遵守方案。这包括频繁更换试剂以及使用“过期”试剂。一个好的H&E染色的适当颜色可以识别许多组织的细微之处，这是准确诊断所必需的。
The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It can be performed manually or by automation. For economic reasons, the manual technique is generally the method of choice for facilities with a low sample volume. This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange. For accurate phenotyping and delineation of tissue detail, the protocol must be adhered to rigorously. This includes frequent reagent changes as well as the use of "in-date" reagents. Appropriate color in a good H&E stain allows for identification of many tissue subtleties that are necessary for accurate diagnosis.