Double strand break repair and γ-H2AX formation in erythrocytes of medaka (Oryzias latipes) after γ-irradiation.
Double strand break repair and γ-H2AX formation in erythrocytes of medaka (Oryzias latipes) after γ-irradiation.
复制标题
γ 辐射后青鳉 (Oryzias latipes) 红细胞中双链断裂修复和 γ-H2AX 形成。
DOI：
10.1016/j.envpol.2016.11.050
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Mitani H
中科院分区：
文献类型：
--
作者：
Sayed AE;Igarashi K;Watanabe-Asaka T;Mitani H
研究γ射线照射后红细胞的DNA损伤反应，可能为γ射线照射作为遗传毒性环境应激的生物标志物提供依据。我们曾报道过青鳉经10戈伊照射后，红细胞出现各种畸形，但未发现微核。在这项研究中，我们优化了15戈伊γ射线照射后成年青鳉鱼红细胞中γ-H2 AX和双链断裂的检测方法。γ射线照射后4 h，细胞凋亡和核异常（包括微核）水平最高，红细胞中γ-H2 AX灶水平最高。这些结果表明，识别和修复过程中发生的红细胞中的DNA损伤的青鳉。
The study of the DNA damage response in erythrocytes after γ-irradiation may provide evidence for its effectiveness as a biomarkers for genotoxic environmental stress. We previously reported various malformations in erythrocytes of medaka irradiated with10 Gy, but not in their micronuclei. In this study, we optimized an assay method for γ-H2AX and double strand breaks in erythrocytes of adult medaka fish after 15 Gy of γ-irradiation. The highest level of apoptosis and nuclear abnormalities, including in micronuclei, were recorded 4 h after γ-irradiation, as was the highest level of γ-H2AX foci in erythrocytes. These results suggest that recognition and repair processes occur as a response to DNA damage in erythrocytes in medaka.