ADRENERGIC INNERVATION OF HUMAN TESTIS EPIDIDYMIS DUCTUS DEFERENS AND PROSTATE - A FLUORESCENCE MICROSCOPIC AND FLUORIMETRIC STUDY
ADRENERGIC INNERVATION OF HUMAN TESTIS EPIDIDYMIS DUCTUS DEFERENS AND PROSTATE - A FLUORESCENCE MICROSCOPIC AND FLUORIMETRIC STUDY
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DOI：
10.1007/bf00496704
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发表时间：
1968-01-01
期刊：
影响因子：
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通讯作者：
OWMAN, T
中科院分区：
文献类型：
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作者：
BAUMGARTEN, HG;FALCK, B;OWMAN, T
1.本文研究了26-79岁病人手术后睾丸、附睾、输精管和前列腺中的儿茶酚胺和5-羟色胺的定位。2.在荧光测定中，发现生殖道中存在不同浓度的去甲肾上腺素。睾丸中含量最低（0.07 μg/g）。在射精管系统的近端方向沿着，去甲肾上腺素水平逐渐增加，在输精管中达到最大值1.43 μg/g。前列腺组织（从增生的腺体中获得）仅含0.15 μg/g去甲肾上腺素，不含多巴胺。肾上腺素很可能是一种假象，仅在一例手术前给予这种胺作为局部麻醉混合物的一部分的组织中出现。3.荧光显微镜分析福尔马林处理的生殖器官显示存在肾上腺素能神经，其数量与去甲肾上腺素浓度一致。附睾、输精管和前列腺中的大量肾上腺素能神经末梢支配平滑肌被，4.组织切片中甲醛诱导荧光的显微分光光度特征证实了肾上腺素能神经含有去甲肾上腺素。5.前列腺中荧光神经节细胞的发现表明，如果不是全部的话，人类的男性生殖道也是通过所谓的短肾上腺素能神经元受神经支配的，所述短肾上腺素能神经元起源于位于外周的交感神经节。6.壶状细胞，形态上类似于肠嗜铬细胞并发射黄色吲哚荧光，存在于前列腺粘膜的上皮中。7.除了特异性的、甲醛诱导的单胺荧光，在上皮层和结缔组织中均出现大量不同颜色和与不同结构相关的自发荧光。
1.The localization of catecholamines and 5-hydroxytryptamine has been investigated in the human testis, epididymis, ductus deferens and prostate obtained at operations on patients aged 26–79 years.2.In fluorimetric determinations noradrenaline was found to be present in varying concentrations in the genital tract. The lowest amount (0.07 μg/g) was found in the testis. In proximal direction along the ejaculatory duct system there was a progressive increase in the noradrenaline level, reaching a maximum of 1.43 μg/g in ductus deferens. The prostatic tissue (obtained from hyperplastic glands) contained only 0.15 μg/g noradrenaline.No dopamine was present. Adrenaline occurred, by all probability as an artefact, only in tissues from one single case to which this amine had been administered as part of the local anaesthetic mixture given before operation.3.Fluorescence microscopic analysis of formaldehyde-treated genital organs revealed the presence of adrenergic nerves, the number of which well agreed with the noradrenaline concentrations.Only vascular nerves were found in the testis. The bulk of the adrenergic nerve terminals in the epididymis, ductus deferens and prostate innervated the smooth muscle coats, whereas only a smaller portion of the adrenergic nerve population was of vasomotor nature.4.Microspectrophotometric characterization of the formaldehyde-induced fluorescence in the tissue sections confirmed that the adrenergic nerves contained noradrenaline.5.The finding of fluorescent ganglion cells in the prostate indicated that part — if not all — of the male genital tract also in humans is innervated by way of so-called short adrenergic neurons, originating in peripherally located sympathetic ganglia.6.Flask-shaped cells, morphologically resembling the enterochromaffin cells and emitting a yellow indole fluorescence, were present in the epithelium of the prostatic mucosa.7.Apart from the specific, formaldehyde-induced monoamine fluorescence, a considerable amount of autofluorescence of varying colour and related to different structures occurred both in the epithelial layers and in the connective tissue.