Bicontinuous Interfacially Jammed Emulsion Gels (bijels) as Media for Enabling Enzymatic Reactive Separation of a Highly Water Insoluble Substrate
Bicontinuous Interfacially Jammed Emulsion Gels (bijels) as Media for Enabling Enzymatic Reactive Separation of a Highly Water Insoluble Substrate
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DOI：
10.1038/s41598-019-42769-8
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发表时间：
2019-04-24
影响因子：
4.6
通讯作者：
Lee, Daeyeon
中科院分区：
文献类型：
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作者：
Cha, Sanghak;Lim, Hyun Gyu;Lee, Daeyeon
尽管酶是能够在温和条件下以高特异性将各种底物转化成所需产物的有效催化剂，但当底物是水溶性差的时，它们作为催化剂的有效性显著降低。在这项研究中，为了加快疏水底物的酶促转化，我们使用了双连续界面堵塞的乳液凝胶（bijel），它提供了两种不混溶的液体：油和水之间的大界面面积。使用脂肪酶催化的水解三丁酸甘油酯作为一个模型反应，在一个批处理模式，我们表明，bijels可以用作介质，使酶促反应。与搅拌的两相介质相比，Bijel系统的初始反应速率增加了四倍。我们的研究结果表明，bijels是强大的两相反应介质，以加速各种疏水试剂的酶促反应。这项工作还表明，Bijels可以潜在地用作反应介质，以实现连续的反应分离。
Although enzymes are efficient catalysts capable of converting various substrates into desired products with high specificity under mild conditions, their effectiveness as catalysts is substantially reduced when substrates are poorly water-soluble. In this study, to expedite the enzymatic conversion of a hydrophobic substrate, we use a bicontinuous interfacially jammed emulsion gel (bijel) which provides large interfacial area between two immiscible liquids: oil and water. Using lipase-catalyzed hydrolysis of tributyrin as a model reaction in a batch mode, we show that bijels can be used as media to enable enzymatic reaction. The bijel system gives a four-fold increase in the initial reaction rate in comparison to a stirred biphasic medium. Our results demonstrate that bijels are powerful biphasic reaction media to accelerate enzymatic reactions with various hydrophobic reagents. This work also demonstrates that bijels can potentially be used as reaction media to enable continuous reactive separations.