Comparison of DNA restriction fragment length polymorphisms of Nostoc strains in and from cycads.
Comparison of DNA restriction fragment length polymorphisms of Nostoc strains in and from cycads.
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苏铁植物发菜菌株 DNA 限制性片段长度多态性的比较。
DOI：
10.1007/bf00447006
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发表时间：
1989
影响因子：
2.8
通讯作者：
Nierzwicki-Bauer,SA
中科院分区：
文献类型：
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作者：
Lindblad,P;Haselkorn,R;Bergman,B;Nierzwicki-Bauer,SA
从5种苏铁自然种群的珊瑚根部新鲜分离的蓝藻提取DNA，这5种苏铁分别是：墨西哥黑苏铁(墨西哥)、墨西哥墨西哥黑苏铁(C.miciana robusta)、小刺苏铁(Dioon Spinulosum)、呋喃泽兰(Zamia FurFuraceae)和墨西哥苏铁(Zamia FurFuraceae)。斯金内里(哥斯达黎加)。利用Southern杂交技术，以克隆的AnabaenaPCC 7120nifK和glnA基因为探针，比较了这些蓝藻共生菌的限制性内切酶片段长度多态性。5种蓝藻样品与两种探针杂交的DNA片段大小不同，说明不同种类和/或菌株的蓝藻存在共生关系。另一方面，对刚从单个珊瑚根中采集的蓝藻和从同一珊瑚根中分离的三个独立传代的蓝藻进行了类似的比较，结果表明它们很可能是同一种微生物。此外，限制性内切酶图谱的复杂性表明，尽管单一的蓝藻菌株可以在单一苏铁植物的根中占据优势地位，但多个新菌株的混合物可以与单一的苏铁物种相关联。因此，广泛的新菌株似乎与苏铁的珊瑚状根有关。
DNA was prepared from cyanobacteria freshly isolated from coralloid roots of natural populations of five cycad species:Ceratozamia mexicana mexicana(Mexico),C. mexicana robusta(Mexico),Dioon spinulosum(Mexico),Zamia furfuraceae(Mexico) andZ. skinneri(Costa Rica). Using the Southern blot technique and clonedAnabaenaPCC 7120nifK andglnA genes as probes, restriction fragment length polymorphisms of these cyanobacterial symbionts were compared. The five cyanobacterial preparations showed differences in the sizes of their DNA fragments hybridizing with both probes, indicating that different cyanobacterial species and/or strains were in the symbiotic associations. On the other hand, a similar comparison of cyanobacteria freshly collected from a singleEncephalartos altensteiniicoralloid root and from three independently subcultured isolates from the same coralloid root revealed that these were likely to be one and the same organism. Moreover, the complexity of restriction patterns shows that a mixture ofNostocstrains can associate with a single cycad species although a single cyanobacterial strain can predominate in the root of a single cycad plant. Thus, a wide range ofNostocstrains appear to associate with the coralloid roots of cycads.