Construction of red-emitting QD probes and determination of indole-propionic acid binding sites in plant tissues
Construction of red-emitting QD probes and determination of indole-propionic acid binding sites in plant tissues
复制标题
DOI：
10.1039/c3ay42044b
复制
发表时间：
2014-03
影响因子：
3.1
通讯作者：
Ying Yu;Ruo-ying Li;Shuang Wu;Bixia Lin;Yujuan Cao;Xiaogang Hu;Jian-Zhong Wu
中科院分区：
文献类型：
--
作者：
Ying Yu;Ruo-ying Li;Shuang Wu;Bixia Lin;Yujuan Cao;Xiaogang Hu;Jian-Zhong Wu
植物激素影响植物的生长发育进程和对环境的响应。因此，了解造成上述现象的原因具有重要意义。由于缺乏准确、灵敏的检测方法，对植物激素信号转导机制的研究还很不够，而激素与受体结合位点的检测是研究植物激素信号转导的第一步。生长素吲哚丙酸（IPA）是一种重要的植物激素，能促进植物生根和结果。将CdTe/ZnS量子点与IPA偶联，构建了CdTe/ZnS-IPA荧光探针，用于植物组织中IPA结合位点的原位检测。通过改进合成条件，在油浴法制备的CdTe表面包覆ZnS，制备了红色发光的CdTe/ZnS量子点，并通过XRD证实了CdTe/ZnS量子点的核/壳结构。通过荧光光谱、荧光衰减寿命、紫外-可见光谱和DLS验证了偶联的成功。选用偶联率为5.4%的CdTe/ZnS-IPA标记绿豆根组织中IPA结合位点，发现绿豆根组织中IPA结合位点主要集中在内皮层细胞中。CdTe/ZnS-IPA与IPA之间存在着对IPA结合位点的竞争识别关系，表明CdTe/ZnS-IPA对IPA具有生物活性。与发绿光的CdTe-IPA相比，发红光的CdTe/ZnS-IPA可以与植物组织的背景形成对比。总之，该探针具有低毒性、抗植物组织背景的特点，且该方法用于植物组织中IPA结合位点的选择性检测具有简便、直观的优点。
Plant hormones influence plant growth and development progress, and response to the environment. Therefore, it is highly significant to know the reasons that cause the above phenomena. Research on the signal transduction mechanism of hormones in plants is insufficient due to the lack of an accurate and sensitive method to measure it. The detection of binding sites between hormones and receptors is the first step in studying the signal transduction of plant hormones. The auxin indole-propionic acid (IPA) is an important plant hormone and can promote rooting and fruiting in plants. By conjugating CdTe/ZnS QDs with the IPA moiety, we constructed the CdTe/ZnS–IPA fluorescence probes for the in situ detection of IPA binding sites in plant tissues. By improving the synthesis conditions, we capped ZnS outside the oil bath-obtained CdTe to prepare red-emitting CdTe/ZnS QDs, and the core/shell structure of the CdTe/ZnS QDs was proved by XRD. The success of conjugation was verified by fluorescence spectra, fluorescence decay lifetime, UV-vis spectra, and DLS. The CdTe/ZnS–IPA with a 5.4% coupling rate were selected for the labelling of IPA binding sites, which are concentrated in the endodermis cells of mung bean root tissues. The existence of the competitive relationship of recognition for IPA binding sites between CdTe/ZnS–IPA and IPA declared that CdTe/ZnS–IPA possessed biological activity for IPA. The red-emitting CdTe/ZnS–IPA can contrast with the background of plant tissues compared to green-emitting CdTe–IPA. In sum, the novel probe showed low toxicity, was plant tissue background-resistant, and this new method for the selective detection of IPA binding sites in plant tissues has the advantages of convenience and easy visualization.