Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens
Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens
复制标题
DOI：
10.1016/j.foodcont.2013.01.032
复制
发表时间：
2013-08-01
期刊：
影响因子：
6
通讯作者：
Baek, Kwang-Hyun
中科院分区：
文献类型：
--
作者：
Bajpai, Vivek K.;Sharma, Ajay;Baek, Kwang-Hyun
本文报道了三棱树果实精油(CEO)的化学成分，并探讨了其对食源性致病菌的可能的抑菌作用方式。采用微波辅助提取技术，对三尖杉果实进行水蒸气蒸馏，得到三尖杉果实的CTEO。通过气相色谱-质谱联用分析，共鉴定出29种化合物，占挥发油总量的94.46%。对蜡样芽孢杆菌ATCC 13061、金黄色葡萄球菌ATCC 12600、单核细胞增生性李斯特菌ATCC 7644、鼠伤寒沙门氏菌ATCC 43174和大肠埃希菌O157：H7 ATCC 43889等食源性致病菌显示出潜在的抑菌作用。CTEO对供试细菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为250-1000µg/mL。在MIC浓度下，CTEO对供试病原菌的细胞活力也有潜在的抑制作用。扫描电子显微镜分析表明，CTEO对蜡样芽孢杆菌ATCC 13061和E.ColiO157：H7 ATCC 43889的细胞壁有明显的抑制作用。此外，CTEO还揭示了其对膜完整性的作用模式，从细胞外ATP的释放、260 nm吸收物质的损失和钾离子对食源性致病菌的泄漏来证实。这些发现表明，CTEO显示了广泛的抗菌效果，并折衷了其对膜完整性的作用模式。(C)2013爱思唯尔有限公司。保留所有权利。
The present investigation reports on the chemical composition of Cudrania tricuspidata fruit essential oil (CEO) and examines its possible antimicrobial mode of action against food-borne pathogenic bacteria. The CTEO was obtained by hydrodistillation of C tricuspidata fruits using a microwave-assisted extraction technique. Gas chromatography-mass spectrometry analysis of the CTEO resulted in the determination of 29 different compounds, representing 94.46% of the total oil. The CTEO (1000 mu g/disc) showed potential antibacterial effect as diameters of inhibition zones (15.0 +/- 0.1-21.0 +/- 1.0 mm) against the tested food-borne pathogenic bacteria including Bacillus cereus ATCC 13061, Staphylococcus aureus ATCC 12600, Listeria monocytogenes ATCC 7644, Salmonella typhimurium ATCC 43174 and Escherichia coli O157:H7 ATCC 43889. The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentration values of CTEO against the tested bacteria were found in the range of 250-1000 mu g/mL, respectively. Also the CTEO had potential inhibitory effect on the cell Viability of the tested pathogens at MIC concentration. The SEM analysis showed the inhibitory effect of CTEO as confirmed by considerable morphological alterations on the cell wall B. cereus ATCC 13061 and E. coli O157:H7 ATCC 43889. In addition, the CTEO revealed its mode of action on membrane integrity as confirmed by release of extracellular ATP, loss of 260-nm absorbing materials and leakage of potassium ions against food-borne pathogenic bacteria. These findings suggest that CTEO showed a broad-spectrum of antibacterial efficacy and compromise its mode of action on membrane integrity. (C) 2013 Elsevier Ltd. All rights reserved.