Myosin synthesis in embryonic chicken fibroblasts.
Myosin synthesis in embryonic chicken fibroblasts.
复制标题
DOI：
10.1083/jcb.81.1.115
复制
发表时间：
1979-04
期刊：
影响因子：
--
通讯作者：
Blauwiekel PB
中科院分区：
文献类型：
--
作者：
Young RB;Bergen WG;Blauwiekel PB
用[3H]亮氨酸脉冲标记法测定了复制胚鸡皮成纤维细胞中组成型肌球蛋白的合成率。这些细胞以3.2 x 10(3)分子/细胞/分钟的速率合成了200,000道尔顿的肌球蛋白（MHC）重链。此外，从总蛋白含量、MHC百分比、成纤维细胞倍增时间和MHC半衰期计算维持组成MHC/细胞恒定水平所需的MHC合成率的独立估计。计算的速率约为2.9 x 10(3)个分子/细胞/分钟，与测量的速率非常接近。相比之下，在完全激活的肌肉细胞培养中，肌原纤维MHC的合成速率约为2.9 x 10(4)个分子/核/分钟。
The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half- life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.