Rheology of Biological Soft Matter: Fundamentals and Applications
Rheology of Biological Soft Matter: Fundamentals and Applications
复制标题
生物软物质流变学：基础与应用
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
I. Kaneda
中科院分区：
文献类型：
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作者：
I. Kaneda
生物大分子之间的构象和相互作用对生物软物质的力学性质至关重要。在这一章中，从基本的角度来看，在单分子水平上的机械特性的方法和应用，被描述为理解流变学方面的基础。原子力显微镜（AFM）和光镊可以在单分子水平上研究分子的力学性质和相互作用。作为特异性和/或非特异性相互作用的结果的两个分子之间的力可以被确定为两个分子之间的距离的函数。选定的例子，在大分子中的相互作用，突出了基于AFM的力谱的观察。这包括多糖对，例如疏水改性的羟乙基纤维素（HMHEC）之间、蛋白多糖和粘蛋白-藻酸盐之间的相互作用。基于单分子对相互作用，讨论了HMHEC-直链淀粉凝胶和海藻酸钠凝胶的物理交联形成机理。对于较慢的键合形成系统，其可能无法使用正常的动态力光谱，可以应用滑动接触力光谱。对于较慢的解离速率，Dudko-Hummer-Szabo模型和Friddle-Noy-De Yoreo模型可以作为Bell-Evans模型的扩展用于分析。本文还讨论了单根M. Takemasa（）早稻田大学创造科学与工程学院，3-4-1，Okubo Shinjuku，Tokyo，Japan电子邮件：takemasa@physics.soft-matter.org A.N.东英吉利大学药学院，诺维奇研究园，诺维奇，NR 4 7 TJ英国M。挪威科技大学Sletmoen生物技术系，挪威特隆赫姆NO-7491斯托克物理系，挪威科技大学，NO-7491特隆赫姆© Springer Japan 2017 I. Kaneda（ed.），生物软物质流变学，软和生物物质，DOI 10.1007/978-4-431-56080-7_1 3 4 M。Takemasa等人提出了在宏观尺度下获得的力学性能的分子研究和弛豫谱，作为理解宏观尺度和微观尺度下力学性能之间的差距的一种可能的方法。
Conformation and interactions between biological macromolecules are crucial for the mechanical properties of biological soft matter. In this chapter, the method and applications of the mechanical characteristics at the single-molecule level, from a fundamental point of view, are described as basis for understanding aspects of rheology. Atomic force microscope (AFM) and optical tweezers can be applied to investigate mechanical properties and interactions of molecules in the single molecular level. The force between two molecules as a result of specific and/or non-specific interactions can be determined as a function of distance between two molecules. Selected examples for interactions in macromolecules were highlighted based on observations by AFM-based force spectroscopy. This includes polysaccharide pairs such as interactions among hydrophobically modified hydroxyethyl cellulose (HMHEC), between protein polysaccharides and mucin– alginate. The mechanism of physically cross-linked hydrogel formation, HMHEC– amylose gel and alginate gels was also discussed based on single molecular pair interactions. For slower bond formation systems, which may not be capable with normal dynamic force spectroscopy, slide contact force spectroscopy can be applied. For slower dissociation rate, Dudko–Hummer–Szabo model and Friddle–Noy–De Yoreo model can be used for the analysis as an extension of the Bell–Evans model. The relation between characteristic timescale of interaction estimated in the single M. Takemasa ( ) School of Creative Science and Engineering, Waseda University, 3-4-1, Okubo Shinjuku, Tokyo, Japan e-mail: takemasa@physics.soft-matter.org A.N. Round School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ UK M. Sletmoen Department of Biotechnology, The Norwegian University of Science and Technology, NO-7491 Trondheim, Norway B.T. Stokke Department of Physics, The Norwegian University of Science and Technology, NO-7491 Trondheim, Norway © Springer Japan 2017 I. Kaneda (ed.), Rheology of Biological Soft Matter, Soft and Biological Matter, DOI 10.1007/978-4-431-56080-7_1 3 4 M. Takemasa et al. molecular study and relaxation spectra in the mechanical properties obtained at the macroscopic scale is presented as a possible way forward in understanding the gap between the mechanical properties in macroscopic and microscopic scale.
DOI：
10.1164/rccm.2112072
发表时间：
2002-11-15
影响因子：
24.7
作者：
Malhotra, A;Huang, YQ;White, DP
通讯作者：
White, DP