A Rhodopseudomonas palustris nifA* mutant produces H2 from NH4+-containing vegetable wastes
A Rhodopseudomonas palustris nifA* mutant produces H2 from NH4+-containing vegetable wastes
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DOI：
10.1016/j.ijhydene.2012.08.009
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发表时间：
2012-11-01
影响因子：
7.2
通讯作者：
De Philippis, Roberto
中科院分区：
文献类型：
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作者：
Adessi, Alessandra;McKinlay, James B.;De Philippis, Roberto
光生物 H-2 生产过程的研究指向使用低成本底物作为 H-2 生成的碳减少源。这些底物（废水或其他发酵过程产生的废水）通常不仅富含碳，而且富含固定氮。 NH4+ 是紫色非硫细菌中固氮酶介导的 H-2 生产的抑制剂。利用组成型表达固氮酶基因的沼泽红假单胞菌突变株 (NifA*) 来测试含有发酵产物的 NH4+ 在光生物学生产 H-2 中的用途。该菌株在合成培养基和废物衍生的含 NH4+ 培养基上生长。在合成培养基和真正的蔬菜废物衍生培养基中，在高浓度 NH4+ 存在的情况下，nifA* 突变体都能产生 H-2，从而导致比野生型菌株更高的 H-2 水平。因此，这项研究表明，NifA* 菌株非常适合克服自然产生的抑制性 NH4+ 的影响，因为它将农业废物转化为生物燃料。版权所有 (C) 2012，Hydrogen Energy Publications, LLC。由爱思唯尔有限公司出版。保留所有权利。
Research on photobiological H-2 production processes is pointing towards the use of low cost substrates as sources of reduced carbon for H-2 generation.Those substrates (either wastewaters or effluents derived from other fermentation processes) are often rich not only in carbon, but also in fixed nitrogen. NH4+ is an inhibitor of nitrogenase-mediated H-2 production in purple non sulfur bacteria.A Rhodopseudomonas palustris mutant strain (NifA*), which constitutively expresses nitrogenase genes, was utilized in order to test the use of NH4+, containing fermentation products for photobiological production of H-2. The strain was grown on both synthetic and waste-derived NH4+ containing media.The nifA* mutant produced H-2 in the presence of high concentrations of NH4+ both in a synthetic medium and in a real vegetable waste-derived medium resulting in higher H-2 levels than the wild-type strain. Thus, this study demonstrates that the NifA* strain is well suited to overcome the effects of inhibitory naturally occurring NH4+ as it converts agricultural waste into biofuel. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.