Reexamination of the sequence of the sea urchin egg receptor for sperm: implications with respect to its properties.
Reexamination of the sequence of the sea urchin egg receptor for sperm: implications with respect to its properties.
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重新检查海胆卵子精子受体的序列：对其特性的影响。
DOI：
10.1006/dbio.1997.8504
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Lennarz,WJ
中科院分区：
文献类型：
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作者：
Just,ML;Lennarz,WJ
对紫癜性圆梭菌海胆精子卵受体的cDNA克隆的重新检查导致了序列的几个重要变化。利用cDNA末端快速扩增（RACE）和Northern blot分析证实，修正后的氨基酸序列缺少一个经典的信号肽。此外，帧移位导致终止密码子在假定的跨膜结构域之前终止推断的序列，从而将蛋白质截断为889个氨基酸。该受体的氨基酸序列与hsp110蛋白亚家族具有较高的序列相似性。这些关于精子卵子受体初级结构的发现提出了一个重要的问题，即重度糖基化受体定位于卵子细胞外表面和皮质颗粒的机制。
A reexamination of the cDNA clones of theStrongylocentrotus purpuratussea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack a classical signal peptide. In addition, a frame shift resulted in a stop codon terminating the deduced sequence prior to a putative transmembrane domain, thereby truncating the protein to 889 amino acids. The deduced amino acid sequence of the receptor has high sequence similarity to the hsp 110 subfamily of proteins. These findings on the primary structure of the egg receptor for sperm raise important questions concerning the mechanism by which the heavily glycosylated receptor is localized to the extracellular surface of the egg and to the cortical granules.