Biological functions of endophytic bacteria in Robinia pseudoacacia 'Hongsen'.
Biological functions of endophytic bacteria in Robinia pseudoacacia 'Hongsen'.
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红花刺槐内生细菌的生物学功能
DOI：
10.3389/fmicb.2023.1128727
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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内生菌与其宿主植物已经共同进化了很长时间。这种关系使得内生菌被普遍认为是一类特殊的微生物资源。‘泓森槐’是一种抗旱、耐瘠薄的树种，在中国南方和北方均可种植，有效解决了中国“南方桉树，北方杨树”所带来的生态问题。到目前为止，已有关于‘泓森槐’固氮菌及其他内生菌的依赖培养的研究。因此，本研究通过高通量测序和依赖培养的方法详细研究了‘泓森槐’的微生物群落。
本研究利用依赖培养（分离）和不依赖培养的技术检测了‘泓森槐’中微生物的种类和功能多样性。
从‘泓森槐’中共分离出210株菌株。通过原位聚合酶链式反应（IS - PCR）指纹图谱模式，这些菌株被聚类为16个组。对每组代表菌株的16S rRNA基因序列分析表明，这些组属于8个属的16个种，证明了‘泓森槐’内生菌的多样性。芽孢杆菌是所有内生细菌中最常见的属。对‘泓森槐’植物内生细菌以及根际土壤细菌的高通量测序表明，根附近土壤、叶片和根际的细菌种群存在显著差异。与根际相比，内生菌中的微生物丰度降低。我们观察到根和叶具有相似的群落结构。在有根瘤和无根瘤的情况下，‘泓森槐’植株中的中慢生根瘤菌属存在显著差异。
据预测，‘泓森槐’植株内生细菌将在代谢过程中发挥重要作用，例如碳水化合物代谢、氨基酸代谢、膜运输和能量代谢。
Endophytes and their host plants have co-evolved for a very long time. This relationship has led to the general recognition of endophytes as a particular class of microbial resources. R. pseudoacacia ‘Hongsen’ is drought- and barren-resistant species that can be grown in both the north and south of China, efficiently addresses the ecological issues caused by China’s ‘southern eucalyptus and northern poplar. Up to date, cultured-dependent studies are available for the R. pseudoacacia nitrogen-fixing and other endophytes. Therefore, the present research studied the R. pseudoacacia ‘Hongsen,’ microbiome in detail by high-throughput sequencing and culture dependant. This study examined microbial species and functional diversity in Robinia pseudoacacia ‘Hongsen’ using culture-dependent (isolation) and culture-independent techniques. A total of 210 isolates were isolated from R. pseudoacacia ‘Hongsen.’ These isolates were clustered into 16 groups by the In Situ PCR (IS-PCR) fingerprinting patterns. 16S rRNA gene sequence analysis of the representative strain of each group revealed that these groups belonged to 16 species of 8 genera, demonstrating the diversity of endophytes in R. pseudoacacia ‘Hongsen’. ’Bacillus is the most prevalent genus among all the endophytic bacteria. High-throughput sequencing of endophytic bacteria from R. pseudoacacia ‘Hongsen’ of the plant and the rhizosphere soil bacteria showed that the bacterial populations of soil near the root, leaf, and rhizosphere differed significantly. The microbial abundance decreased in the endophytes as compared to the rhizosphere. We observed a similar community structure of roots and leaves. With and without root nodules, Mesorhizobium sp. was significantly different in R. pseudoacacia ‘Hongsen’ plant. It was predicted that R. pseudoacacia ‘Hongsen’ plant endophytic bacteria would play a significant role in the metabolic process, such as carbohydrate metabolism, amino acid metabolism, membrane transport, and energy metabolism.