Identification of a novel effector BxSapB3 that enhances the virulence of pine wood nematode Bursaphelenchus xylophilus
Identification of a novel effector BxSapB3 that enhances the virulence of pine wood nematode Bursaphelenchus xylophilus
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DOI：
10.1093/abbs/gmz100
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发表时间：
2019-10-01
影响因子：
3.7
通讯作者：
Wu, Xiaoqin
中科院分区：
文献类型：
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作者：
Huang, Xin;Hu, Longjiao;Wu, Xiaoqin
松材线虫病是由松材线虫引起的一种严重危害我国松林的病害。在我们之前的研究中，该病原体分泌的效应物被证明在松树枯萎病的不同感染阶段发挥作用，并通过转录组测序预测了一系列候选效应物。这项研究确定并表征了一种新的效应子BxSapB 3，它是这些候选效应子之一。农杆菌介导的瞬时表达用于鉴定BxSapB 3。BxSapB 3由B分泌。在本氏烟草（Nicotiana benthamiana）中，发现能够诱导细胞死亡。实时定量PCR（qRT-PCR）分析显示，BxSapB 3在B的高毒力菌株中上调。在感染的早期阶段，在弱毒力菌株中以较低水平表达。当BxSapB 3在B.感染的过程被推迟。这些结果表明，BxSapB 3作为效应子发挥作用，并在B的早期阶段对毒力有贡献。松材线虫感染
Pine wilt disease, caused by the pine wood nematode Bursaphelenchus xylophilus, leads to severe damage to pine forests in China. In our previous study, effectors secreted by this pathogen were shown to play roles in the different infection stages of pine wilt disease, and a series of candidate effectors were predicted by transcriptome sequencing. This study identified and characterized a novel effector, BxSapB3, which was among these candidate effectors. Agrobacterium-mediated transient expression was used to identify BxSapB3. BxSapB3 was secreted by B. xylophilus and found to be capable of inducing cell death in Nicotiana benthamiana. Quantitative real-time PCR (qRT-PCR) analysis revealed that BxSapB3 was upregulated in a highly virulent strain of B. xylophilus and expressed at lower levels in a weakly virulent strain at the early stages of infection. When BxSapB3 was silenced in B. xylophilus, the process of infection was delayed. These results indicate that BxSapB3 acts as an effector and contributes to virulence at the early stages of B. xylophilus infection.