Molecular Evidence of Different Rickettsia Species in Villeta, Colombia
Molecular Evidence of Different Rickettsia Species in Villeta, Colombia
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DOI：
10.1089/vbz.2015.1841
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发表时间：
2016-02-01
影响因子：
2.1
通讯作者：
Hidalgo, Marylin
中科院分区：
文献类型：
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作者：
Faccini-Martinez, Alvaro A.;Ramirez-Hernandez, Alejandro;Hidalgo, Marylin
这项工作的目的是检测和鉴定在哥伦比亚比列塔农村地区收集的蜱中的立克次体。蜱虫标本采集自Villeta镇五个农村村庄的家畜和房屋墙壁，以及Naranjal村（同一城镇）的人类。此外，还处理了从同一地区收集的跳蚤。提取DNA并通过常规、半巢式和巢式PCR反应检测立克次体基因。在从Naranjal村的人类收集的蜱中，使用ompA的引物扩增了广义上的卡氏钝眼蜱的若虫并测序（与“钝眼立克次体”100%同一性）。最后，从猫栉首蚤中扩增出的gltA、ompB和16SrRNA基因的3个片段与R.猫立克次体（分别为98.5%、97.3%和99.2%）和“阿氏立克次体”（分别为99.7%和100%）。据我们所知，这些结果对应于哥伦比亚首次分子检测到的“安氏立克次体”和“Ca.在跳蚤体内发现了“阿斯彭立克次体”。
The aim of this work was to detect and identify Rickettsia species in ticks collected in rural areas of Villeta, Colombia. Tick specimens were collected from domestic animals and walls of houses in five rural villages of Villeta town and from humans in Naranjal village (same town). Moreover, a flea collected from the same area was also processed. DNA was extracted and tested by conventional, semi-nested, and nested PCR reactions targeting rickettsial genes. In the ticks collected from humans from Naranjal village, a nymph of Amblyomma cajennense sensu lato was amplified using primers for ompA and sequenced (100% identity with "Candidatus Rickettsia amblyommii''). Last, three amplicons from the Ctenocephalides felis flea, corresponding to gltA, ompB, and 16S rRNA genes, showed high identity with R. felis (98.5%, 97.3%, and 99.2%, respectively) and "Candidatus Rickettsia asemboensis'' (99.7% and 100%, respectively). To our knowledge, these results correspond to the first molecular detection in Colombia of "Candidatus Rickettsia amblyommii'' and "Ca. Rickettsia asemboensis'' in fleas.