SPECTROPHOTOMETRIC ANALYSES OF RIBOSE AND 2-DEOXY-D-RIBOSE ALONE AND IN MIXTURES
SPECTROPHOTOMETRIC ANALYSES OF RIBOSE AND 2-DEOXY-D-RIBOSE ALONE AND IN MIXTURES
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DOI：
10.1002/jps.2600561206
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发表时间：
1967-01-01
影响因子：
3.8
通讯作者：
SEYDEL, JK
中科院分区：
文献类型：
--
作者：
GARRETT, ER;BLANCH, J;SEYDEL, JK
在热和酸性条件下产生的核糖（R）发色团的产生速率和2-脱氧-D-核糖（DR）发色团的最大吸光度与其糖浓度直接相关。这些因素是对这些糖进行定性和定量分析的基础。[lambda]max。在80.0[度]下用1.0 M HCl处理5小时后，降解DR的261 m[mu]发色团稳定至少15小时。降解R的[lambda]max 277 m [mu]发色团在同一时期内以表观零级速率连续增加。这些现象作为一个敏感的分光光度法测定两种糖，单独和混合物的基础。这些酸产生的发色团在碱性时产生具有特定性质的其他吸收带，这些吸收带允许在糖之间进行区分的其他方法。
The rates of production of the chromophore for ribose (R) and the maximum absorbance of the chromophore for 2-deoxy-D-ribose (DR), produced under thermal and acidic conditions are directly related to their sugar concentrations. These factors are the bases for qualitative and quantitative analysis of these sugars. The [lambda]max. 261 m[mu] chromophore of degraded DR is stable for at least 15 hr. after 5 hr. of treatment at 80.0[degree] with 1.0 M HC1. The [lambda]max 277m[mu] chromophore of degraded R increases continuously during the same period at an apparent zero-order rate. These phenomena serve as bases for a sensitive spectro-photometric assay of both sugars, alone and in mixtures. These acid-generated chromophores when made alkaline generate other absorption bands with specific properties which permit additional methods of differentiation between the sugars.