Generation of self-incompatible Arabidopsis thaliana by transfer of two S locus genes from A-lyrata
Generation of self-incompatible Arabidopsis thaliana by transfer of two S locus genes from A-lyrata
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DOI：
10.1126/science.1072205
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发表时间：
2002-07-12
期刊：
影响因子：
56.9
通讯作者：
Nasrallah, JB
中科院分区：
文献类型：
--
作者：
Nasrallah, ME;Liu, P;Nasrallah, JB
在自然和驯化的植物种群中经常发生从异交交配到自交交配的转变，但其潜在的遗传原因尚不清楚。我们发现，柱头受体激酶SRK和其花粉携带的配体SCR从一个S-位点单倍型的自交不亲和和交叉杂交拟南芥lyrata的基因转移是足以赋予自交不亲和表型在自交能育的拟南芥，其中缺乏这些基因的功能直系同源物。这种成功的互补表明，导致抑制自交花粉的信号级联反应在A. thaliana.自交不亲和性的分析将有助于在这个物种的工具。
Transitions from cross-fertilizing to self-fertilizing mating systems have occurred frequently in natural and domesticated plant populations, but the underlying genetic causes are unknown. We show that gene transfer of the stigma receptor kinase SRK and its pollen-borne ligand SCR from one S-locus haplotype of the self-incompatible and cross-fertilizing Arabidopsis lyrata is sufficient to impart self-incompatibility phenotype in self-fertile Arabidopsis thaliana, which lacks functional orthologs of these genes. This successful complementation demonstrates that the signaling cascade leading to inhibition of self-related pollen was maintained in A. thaliana. Analysis of self-incompatibility will be facilitated by the tools available in this species.