In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration.
In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration.
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DOI：
10.1016/j.colsurfb.2016.10.037
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Weiling Yu;Huakun Zhao;Zhenyu Ding;Zhiwang Zhang;Benben Sun;Ji Shen;Shanshan Chen;Bingchun Zhang-Bingchu
中科院分区：
文献类型：
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作者：
Weiling Yu;Huakun Zhao;Zhenyu Ding;Zhiwang Zhang;Benben Sun;Ji Shen;Shanshan Chen;Bingchun Zhang-Bingchu
多孔镁支架因其可降解性和良好的力学性能而受到越来越多的关注。本文采用激光打孔技术制备了多孔可降解AZ 31镁合金支架。为了提高AZ 31支架的耐腐蚀性和细胞相容性，采用氟化处理获得MgF 2涂层。通过浸泡和电化学测试证实了增强的耐腐蚀性。由于MgF 2涂层提供的保护，FAZ 31支架降解导致的镁释放和pH升高是可控的。此外，体外研究表明，与AZ 31支架相比，MgF 2涂层AZ 31（FAZ 31）支架增强了大鼠骨髓基质细胞（rBMSC）的增殖和附着。此外，我们目前的数据表明，FAZ 31支架提取物可以增强rBMSCs的成骨分化。为比较AZ 31和FAZ 31支架的体内再生能力，使用兔股骨髁缺损模型。显微计算机断层扫描（micro-CT）和组织学检查，以评估降解的支架和骨体积的变化。除了增强的耐腐蚀性外，FAZ 31支架具有更好的生物相容性，并在体内诱导更多的新骨形成。相反，从AZ 31支架观察到骨吸收。这些有希望的结果表明，氟预处理的AZ 31支架骨组织修复和再生的潜在临床应用。
Porous magnesium scaffolds are attracting increasing attention because of their degradability and good mechanical property. In this work, a porous and degradable AZ31 magnesium alloy scaffold was fabricated using laser perforation technique. To enhance the corrosion resistance and cytocompatibility of the AZ31 scaffolds, a fluoride treatment was used to acquire the MgF2coating. Enhanced corrosion resistance was confirmed by immersion and electrochemical tests. Due to the protection provided by the MgF2coating, the magnesium release and pH increase resulting from the degradation of the FAZ31 scaffolds were controllable. Moreover,in vitrostudies revealed that the MgF2coated AZ31 (FAZ31) scaffolds enhanced the proliferation and attachment of rat bone marrow stromal cells (rBMSCs) compared with the AZ31 scaffolds. In addition, our present data indicated that the extract of the FAZ31 scaffold could enhance the osteogenic differentiation of rBMSCs. To compare thein vivobone regenerative capacity of the AZ31 and FAZ31 scaffolds, a rabbit femoral condyle defect model was used. Micro-computed tomography (micro-CT) and histological examination were performed to evaluate the degradation of the scaffolds and bone volume changes. In addition to the enhanced the corrosion resistance, the FAZ31 scaffolds were more biocompatible and induced significantly more new bone formationin vivo. Conversely, bone resorption was observed from the AZ31 scaffolds. These promising results suggest potential clinical applications of the fluoride pretreated AZ31 scaffold for bone tissue repair and regeneration.