Intriguing diversity of Bacillus anthracis in eastern Poland -: the molecular echoes of the past outbreaks
Intriguing diversity of Bacillus anthracis in eastern Poland -: the molecular echoes of the past outbreaks
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DOI：
10.1016/j.femsle.2004.08.038
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发表时间：
2004-10-15
影响因子：
2.1
通讯作者：
Rastawicki, W
中科院分区：
文献类型：
--
作者：
Gierczynski, R;Kaluzewski, S;Rastawicki, W
多位点VNTRs分析（MLVA）显示，存在5种基因型的一组10个炭疽芽孢杆菌分离物的流行病学无关的牛炭疽病例在波兰东部。八个测试的分离株具有pagA和capB基因，表明存在两个毒力质粒，而两个分离株显示只有pagA和缺乏pXO2。MLVA和DNA序列分析表明，7个测试菌株代表4个新的基因型。5个供试菌株显示了一个独特的144 bp的vrrB2变体以及220 bp的vrrB1变体，这意味着与谱系B2的相关性。因此，我们建议建立新的132菌株亚系。几十年前在波兰发生的多次炭疽疫情被认为是B有趣多样性的原因。在这项研究中观察到的炭疽病。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
The multiple locus VNTRs analysis (MLVA) revealed the presence of five genotypes in a group of 10 Bacillus anthracis isolates from epidemiologically unrelated cases of bovine-anthrax in eastern Poland. Eight tested isolates possessed the pagA and capB genes indicating the presence of both virulence plasmids, while two isolates revealed only pagA and lacked pXO2. The MLVA and DNA sequence analysis indicated that seven tested isolates represent four novel genotypes. Five tested strains revealed a unique 144 bp vrrB2 variant as well as 220 bp variant of vrrB1, implying the relatedness to the lineage B2. Consequently, we propose establishing of novel 132 strains sub-lineage. Multiple anthrax outbreaks, which took place in Poland several decades ago were proposed as a cause of intriguing diversity of B. anthracis observed in this study. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.