Heme-iron plays a key role in the regulation of the Ess/Type VII secretion system of Staphylococcus aureus RN6390
Heme-iron plays a key role in the regulation of the Ess/Type VII secretion system of Staphylococcus aureus RN6390
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血红素铁在金黄色葡萄球菌 RN6390 Ess/VII 型分泌系统的调节中发挥关键作用
DOI：
10.1101/145433
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Casabona M
中科院分区：
文献类型：
--
作者：
Casabona M
金黄色葡萄球菌VII型蛋白分泌系统（T7SS）在致病性和种内竞争中起重要作用。在这里，我们表明，在菌株RN 6390中的T7 SS被激活的生长培养基中补充血红蛋白，其辅因子血红素（血红素B）。转录分析和分泌测定表明，激活血红素发生在转录和翻译后水平。缺失essC导致T7分泌活性的丧失，导致铁获得所需基因的上调。总之，这些研究结果表明，T7SS在铁稳态中起作用，至少在一些。金黄色菌株。
TheStaphylococcus aureustype VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion ofessCresults in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least someS. aureusstrains.