Comparison of enzymatic and non-enzymatic glucose sensors based on hierarchical Au-Ni alloy with conductive polymer
Comparison of enzymatic and non-enzymatic glucose sensors based on hierarchical Au-Ni alloy with conductive polymer
复制标题
DOI：
10.1016/j.bios.2019.01.028
复制
发表时间：
2019-04-01
影响因子：
12.6
通讯作者：
Shim, Yoon-Bo
中科院分区：
文献类型：
--
作者：
Lee, Won-Chul;Kim, Kwang-Bok;Shim, Yoon-Bo
制备了基于纳米结构金镍合金的酶促和非酶促安培葡萄糖传感器并比较了它们的性能。分级结构的Au-Ni表面仅用于非酶促葡萄糖传感器，而在合金表面形成的葡萄糖氧化酶附着的聚-3'（苯甲酸）-2,2':5',2'-三噻吩（pTBA）被用作酶促传感器。使用表面分析和电化学实验对所制造的传感器进行了表征。在酶传感器中，酶反应产生的 H2O2 阳极电流被用作分析信号，而在没有固定酶的纯金镍合金电极上观察到葡萄糖的直接氧化，即使在生理 pH 值下也显示出优异的葡萄糖催化氧化作用。传感器表面的电位脉冲预处理提高了性能，使传感器具有可重复性和可重复使用性（酶传感器：变异系数 = 1.82%，n = 5，非酶传感器：变异系数 = 2.93%）。与非酶传感器相比，酶生物传感器具有更高的灵敏度、选择性和稳定性的优点。酶传感器的线性范围为1.0 μM至30.0 mM，检测限为0.29 μM。还通过人体血液样本中的葡萄糖分析证明了传感器的可靠性，并将结果与市售血糖仪进行了比较。
Enzymatic and non-enzymatic amperometric glucose sensors based on nanostructured Au-Ni alloy were prepared and compared in their performance. The hierarchically structured Au-Ni surface was merely used for the non-enzymatic glucose sensor, while glucose oxidase attached poly-3'(benzoic acid) -2,2':5',2'- terthiophene (pTBA) formed on the alloy surface was used as the enzymatic sensor. The fabricated sensor was characterized using surface analysis and electrochemical experiments. In case of the enzymatic sensor, the anodic current of H2O2 generated from the enzyme reaction was used as the analytical signal, while the direct oxidation of glucose was observed on a mere Au-Ni alloy electrode without enzyme immobilization, which shows an excellent catalytic oxidation of glucose even in physiological pH. The potential pulse pretreatment of the sensor surfaces improved the performance, which allowed both the sensors reproducible and reusable (enzymatic sensor: coefficient of variation = 1.82%, n = 5, non-enzymatic: coefficient of variation = 2.93%). The enzymatic biosensor reveals the advantages of increased sensitivity, selectivity, and stability, compared with the non-enzymatic sensor. The linear range of enzymatic sensor was attained from 1.0 mu M to 30.0 mM with a detection limit of 0.29 mu M. The reliabilities of the sensors were also demonstrated through the glucose analysis in human blood samples, and the result was compared with the commercially available glucometer.